HLA-G Antibody HLAG/6686 | MHC Class I Antigen G

	Catalog No	Formulation	Size	Price (USD)
	V8991-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide	100 ug	559
Formulation
	V8991-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide	20 ug	259
Formulation
	V8991SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	559
Formulation

HLA-G Antibody HLAG/6686 Staining of Human Tonsil Lymphoid Tissue IHC. Immunohistochemistry of FFPE human tonsil tissue using HLA-G Antibody clone HLAG/6686 at 2 ug/ml. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris containing 1 mM EDTA, pH 9, for 20 min followed by cooling before testing. Strong HLA-G immunoreactivity is observed across a broad population of cells within the lymphoid tissue.

HLA-G Antibody HLAG/6686 Staining of Human Prostate Epithelium IHC. Immunohistochemistry of FFPE human prostate tissue using HLA-G Antibody clone HLAG/6686 at 2 ug/ml. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris containing 1 mM EDTA, pH 9, for 20 min followed by cooling before testing. HLA-G staining is observed in glandular epithelial cells. The inset shows the negative control prepared with PBS in place of the primary antibody.

HLA-G Antibody HLAG/6686 Detection in Raji B Lymphoma Cells WB. Western blot analysis of human Raji B lymphoma cell lysate using HLA-G Antibody clone HLAG/6686. A strong immunoreactive band is detected at approximately 40-45 kDa, consistent with the expected molecular weight range of HLA-G. The result demonstrates detection of endogenous MHC Class I Antigen G in human Raji cells.

MHC Class I Antigen G Staining in Human Tonsil with HLAG/6686 IHC. Immunohistochemistry of FFPE human tonsil tissue using HLA-G Antibody clone HLAG/6686 at 2 ug/ml. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris containing 1 mM EDTA, pH 9, for 20 min followed by cooling before testing. Widespread HLA-G immunoreactivity is visible within the lymphoid compartment, with variation in staining intensity among individual cells.

HLA-G Antibody HLAG/6686 Detection in JEG-3 Choriocarcinoma Cells WB. Western blot analysis of human JEG-3 placental choriocarcinoma cell lysate using HLA-G Antibody clone HLAG/6686. A strong immunoreactive band is detected at approximately 36-38 kDa, consistent with the expected molecular weight of HLA-G. The result demonstrates detection of endogenous HLA-G in JEG-3 cells, a trophoblast-derived cancer cell line commonly used to study HLA-G expression.

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HLA-G Antibody HLAG/6686 Staining of Human Tonsil Lymphoid Tissue IHC. Immunohistochemistry of FFPE human tonsil tissue using HLA-G Antibody clone HLAG/6686 at 2 ug/ml. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris containing 1 mM EDTA, pH 9, for 20 min followed by cooling before testing. Strong HLA-G immunoreactivity is observed across a broad population of cells within the lymphoid tissue.

HLA-G Antibody HLAG/6686 Staining of Human Prostate Epithelium IHC. Immunohistochemistry of FFPE human prostate tissue using HLA-G Antibody clone HLAG/6686 at 2 ug/ml. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris containing 1 mM EDTA, pH 9, for 20 min followed by cooling before testing. HLA-G staining is observed in glandular epithelial cells. The inset shows the negative control prepared with PBS in place of the primary antibody.

HLA-G Antibody HLAG/6686 Detection in Raji B Lymphoma Cells WB. Western blot analysis of human Raji B lymphoma cell lysate using HLA-G Antibody clone HLAG/6686. A strong immunoreactive band is detected at approximately 40-45 kDa, consistent with the expected molecular weight range of HLA-G. The result demonstrates detection of endogenous MHC Class I Antigen G in human Raji cells.

MHC Class I Antigen G Staining in Human Tonsil with HLAG/6686 IHC. Immunohistochemistry of FFPE human tonsil tissue using HLA-G Antibody clone HLAG/6686 at 2 ug/ml. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris containing 1 mM EDTA, pH 9, for 20 min followed by cooling before testing. Widespread HLA-G immunoreactivity is visible within the lymphoid compartment, with variation in staining intensity among individual cells.

HLA-G Antibody HLAG/6686 Detection in JEG-3 Choriocarcinoma Cells WB. Western blot analysis of human JEG-3 placental choriocarcinoma cell lysate using HLA-G Antibody clone HLAG/6686. A strong immunoreactive band is detected at approximately 36-38 kDa, consistent with the expected molecular weight of HLA-G. The result demonstrates detection of endogenous HLA-G in JEG-3 cells, a trophoblast-derived cancer cell line commonly used to study HLA-G expression.

Availability	1-3 business days
Species Reactivity	Human
Format	Purified
Host	Mouse
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG1, kappa
Clone Name	HLAG/6686
Purity	Protein A/G affinity
UniProt	P17693
Localization	Cell surface
Applications	Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations	This HLA-G Antibody HLAG/6686 / MHC Class I Antigen G is available for research use only.
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Description

HLA-G Antibody HLAG/6686 / MHC Class I Antigen G detects human leukocyte antigen G (HLA-G), a non-classical major histocompatibility complex class I molecule with important roles in immune regulation and tolerance. Unlike the broadly expressed classical HLA-A, HLA-B and HLA-C molecules, HLA-G has a relatively restricted physiological expression pattern. It was initially characterized in placental trophoblasts and is particularly associated with the maternal-fetal interface, where modulation of maternal immune responses contributes to tolerance of the developing fetus.

HLA-G shares the basic structural organization of MHC class I proteins and can associate with beta-2 microglobulin. Alternative splicing of HLA-G transcripts generates multiple membrane-bound and soluble isoforms, adding complexity to its immunoregulatory functions. Rather than functioning primarily as a conventional antigen-presenting molecule, HLA-G is recognized for its ability to influence both innate and adaptive immune responses. This distinctive biology has established MHC Class I Antigen G as an important target for studies of immune tolerance, pregnancy and immune-mediated disease.

HLA-G can regulate immune activity through interactions with inhibitory leukocyte immunoglobulin-like receptors, particularly LILRB1 (ILT2) and LILRB2 (ILT4). These receptors are distributed across multiple lymphoid and myeloid populations, allowing HLA-G to influence NK cells, T cells, B cells, monocytes, macrophages and antigen-presenting cells. HLA-G has also been studied in relation to KIR2DL4 on NK cells, although the biology of this interaction is complex. Through these receptor systems, HLA-G can contribute to suppression or modulation of cytotoxic and inflammatory immune responses.

The tolerogenic properties of HLA-G have generated considerable interest beyond reproductive immunology. Altered or induced HLA-G expression has been investigated in transplantation, inflammatory and infectious diseases, and cancer. In tumors, HLA-G expression is of particular interest as a potential mechanism of immune escape because suppression of immune effector activity may help malignant cells evade immune surveillance. HLA-G is therefore increasingly studied alongside immune checkpoint and other immunoregulatory molecules when examining interactions between tumor cells and the immune microenvironment.

Clone HLAG/6686 is a mouse monoclonal antibody directed against HLA-G. Its recognition of this non-classical MHC class I molecule provides a research reagent for investigating HLA-G protein expression and the biological contexts in which this immunoregulatory molecule is present. The HLA-G Antibody can complement antibodies to other HLA molecules and immune regulatory proteins when examining antigen presentation, immune recognition and mechanisms of immune tolerance.

Researchers investigating HLA-G-mediated immune regulation and related immune pathways can explore additional targets on our Immunology Antibodies page. NSJ Bioreagents provides antibodies targeting HLA molecules and other proteins involved in immune recognition and regulation. An HLA-G Antibody provides a research tool for detecting MHC Class I Antigen G and investigating its roles in maternal-fetal tolerance, immune regulation and cancer biology.

Researchers investigating HLA-G-mediated immune regulation and related immune pathways can explore additional targets on our Immunology Antibodies page.

Application Notes

Optimal dilution of the HLA-G Antibody HLAG/6686 / MHC Class I Antigen G should be determined by the researcher.

Immunogen

Purified His-tagged HLA-G protein was used as the immunogen for the HLA-G antibody.

Storage

Aliquot the HLA-G antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Alternate Names

HLAG antibody, MHC class I antigen G antibody, HLA class I histocompatibility antigen alpha chain G antibody, HLA-G histocompatibility antigen antibody

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HLA-G Antibody HLAG/6686 / MHC Class I Antigen G [clone HLAG/6686] (V8991)

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