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Home >> Antibodies >> HLA-DRB1 Antibody (MHC II)

HLA-DRB1 Antibody (MHC II) [clone SPM289] (V2587)

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  Catalog No Formulation Size Price (USD)  
Image V2587-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V2587-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V2587SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V2587IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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Immunofluorescent staining of human Raji cells with HLA-DRB1 antibody (clone SPM289, green) and Reddot nuclear stain (red).
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Immunofluorescent staining of human Raji cells with HLA-DRB1 antibody (clone SPM289, green) and Reddot nuclear stain (red).
Flow cytometry testing of human Raji cells with HLA-DRB1 antibody (clone SPM289); Red=isotype control, Blue= HLA-DRB1 antibody.
IHC staining of FFPE human tonsil with HLA-DRB1 antibody (clone SPM289). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Western blot testing of human spleen and Ramos lysate with HLA-DRB1 antibody (clone SPM289). Predicted molecular weight ~30 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name SPM289
Purity Protein G affinity chromatography
UniProt P01911
Localization Cell surface
Applications Flow Cytometry : 1-2ug/million cells in 0.1ml
Immunofluorescence : 2-4ug/ml
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This HLA-DRB1 antibody is available for research use only.
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Description

This mAb reacts with the beta-chain of HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The SPM289 antibody recognizes a different epitope than the L243 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.

Application Notes

Optimal dilution of the HLA-DRB1 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Activated human peripheral blood mononuclear cells were used as the immunogen for the HLA-DRB1 antibody.

Storage

Store the HLA-DRB1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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