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Home >> Antibodies >> HLA-DRB1 Antibody for IF SPM289 / HLA-DRB1 Immunofluorescence Antibody

HLA-DRB1 Antibody for IF SPM289 / HLA-DRB1 Immunofluorescence Antibody [clone SPM289] (V2587)

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  Catalog No Formulation Size Price (USD)  
Image V2587-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 559
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V2587-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 259
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V2587SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 559
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V2587IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 559
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HLA-DRB1 Antibody for IF SPM289 immunofluorescence analysis of human cells. Immunofluorescent staining of human Raji cells using HLA-DRB1 Antibody for IF clone SPM289 reveals strong membranous and peripheral cell surface fluorescence consistent with expression of the HLA-DR beta chain / HLA-DRB1 on antigen-presenting B lymphocytes. Cells were labeled with the mouse monoclonal antibody followed by a fluorescent secondary antibody producing green signal, while nuclei are counterstained with Reddot (red). The staining pattern highlights the characteristic plasma membrane localization of MHC class II HLA-DR molecules involved in peptide antigen presentation.
Flow cytometry testing of human Raji cells with HLA-DRB1 antibody (clone SPM289); Red=isotype control, Blue= HLA-DRB1 antibody.
IHC staining of FFPE human tonsil with HLA-DRB1 antibody SPM289. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Western blot testing of human spleen and Ramos lysate with HLA-DRB1 antibody (clone SPM289). Predicted molecular weight ~30 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name SPM289
Purity Protein G affinity chromatography
UniProt P01911
Localization Cell surface
Applications Flow Cytometry : 1-2ug/million cells in 0.1ml
Immunofluorescence : 2-4ug/ml
Western Blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC Only Format : incubate for 30 min at RT (1)
Limitations This HLA-DRB1 antibody is available for research use only.
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Description

Major histocompatibility complex class II DR beta 1 (HLA-DRB1) is a transmembrane glycoprotein encoded by the HLA-DRB1 gene and forms the beta chain of the HLA-DR antigen receptor responsible for peptide antigen presentation to CD4-positive T lymphocytes. HLA-DRB1 Antibody for IF enables visualization of the HLA-DR beta chain in cells using immunofluorescence microscopy, allowing researchers to examine the distribution and cellular localization of major histocompatibility complex class II molecules within antigen-presenting immune cells.

The HLA-DR receptor is a heterodimeric complex composed of an alpha chain encoded by HLA-DRA paired with a beta chain encoded by HLA-DRB genes. This receptor binds peptides derived from extracellular proteins that have been processed within endosomal compartments and transports these peptide antigens to the cell surface for presentation to CD4-positive helper T lymphocytes. Through this mechanism, the HLA-DR complex plays a central role in adaptive immune responses and immune surveillance. Because of this function, HLA-DR expression is characteristic of professional antigen-presenting cells including B lymphocytes, macrophages, and dendritic cells.

HLA-DRB1 represents the primary beta chain gene of the HLA-DR complex and is highly polymorphic across human populations. Numerous HLA-DRB1 allelic variants contribute to diversity in peptide binding and antigen recognition, influencing immune responses and susceptibility to autoimmune diseases. Detection of HLA-DR beta chain expression is therefore widely used in immunology research to study antigen-presenting cell populations and immune activation pathways.

Immunofluorescence analysis provides a powerful method for visualizing HLA-DRB1 expression and distribution within individual cells. Fluorescent antibody labeling allows detection of the HLA-DR beta chain at the plasma membrane and within intracellular compartments associated with antigen processing and presentation. Antibodies such as mouse monoclonal clone SPM289 support immunofluorescence studies examining localization of MHC class II molecules, cellular immune activation, and antigen presentation dynamics in cultured cells and experimental systems.

Application Notes

Optimal dilution of the HLA-DRB1 Antibody for IF SPM289 should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Activated human peripheral blood mononuclear cells were used as the immunogen for the HLA-DRB1 antibody.

Storage

Store the HLA-DRB1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

HLA-DR beta antibody, HLA-DRB1 antibody, MHC class II DR beta antibody, HLA class II histocompatibility antigen DR beta antibody

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