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Home >> Antibodies >> HLA-DRB1 Antibody (MHC II)

HLA-DRB1 Antibody (MHC II) [clone LN3] (V2586)

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  Catalog No Formulation Size Price (USD)  
Image V2586-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 539
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V2586-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 249
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V2586SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
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V2586IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 539
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Immunofluorescent staining of human Ramos cells with HLA-DRB1 antibody (clone LN3, green) and Reddot nuclear stain (red).
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Immunofluorescent staining of human Ramos cells with HLA-DRB1 antibody (clone LN3, green) and Reddot nuclear stain (red).
IHC staining of FFPE human tonsil tissue with HLA-DRB1 antibody (clone LN3). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human histiocytoma tissue with HLA-DRB1 antibody (clone LN3). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human Ramos cell lysate with HLA-DRB1 antibody (clone LN3). Expected molecular weight ~30kDa.
Western blot testing of human Ramos and spleen cell lysate with HLA-DRB1 antibody (clone LN3). Expected molecular weight ~30kDa.
Flow cytometry staining of human Raji cells with HLA-DRB1 antibody; Red=isotype control, Blue= HLA-DRB1 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name LN3
Purity Protein G affinity chromatography
UniProt P01911
Localization Cytoplasmic, membranous
Applications Flow Cytometry : 1-2ug/10^6 cells
Immunofluorescence : 1-3ug/ml
Western Blot : 2-4ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This HLA-DRB1 antibody is available for research use only.
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Description

HLA-DRB1 binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. [UniProt]

Application Notes

Optimal dilution of the HLA-DRB1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Activated human peripheral blood mononuclear cells were used as the immunogen for the HLA-DRB1 antibody.

Storage

Store the HLA-DRB1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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