HLA-DR Antibody Clone LN3 [clone LN3] (V2586)

Description

Human leukocyte antigen DR alpha (HLA-DRA) is a major histocompatibility complex class II protein encoded by the HLA-DRA gene and forms the alpha chain of the HLA-DR antigen presentation receptor expressed on antigen-presenting immune cells. HLA-DR Antibody Clone LN3 recognizes the HLA-DR antigen complex, a widely used immunologic marker of major histocompatibility complex class II expression on B lymphocytes, dendritic cells, macrophages, and activated lymphocytes.

HLA-DR is a heterodimeric receptor composed of an alpha chain encoded by HLA-DRA and a beta chain encoded by HLA-DRB genes. This receptor binds peptides derived from extracellular proteins that have been processed within endosomal compartments and presents them at the cell surface to CD4-positive T lymphocytes. Through this antigen presentation pathway, the HLA-DR complex plays a central role in adaptive immune activation and immune surveillance. Because of its critical function in immune recognition, HLA-DR expression is commonly used as a marker of professional antigen-presenting cells and activated immune populations.

In immunology literature the HLA-DR receptor complex is often referred to simply as HLA-DR, and antibodies targeting this molecule are frequently described as HLA-DR antibodies, HLA-DR alpha antibodies, or MHC class II DR antibodies. HLA-DR antibody reagents therefore detect the HLA-DR antigen complex present on antigen-presenting cells and immune infiltrates within tissues. Expression of HLA-DR alpha can be strongly induced by inflammatory cytokines such as interferon-gamma, resulting in increased MHC class II expression during infection, autoimmune disease, inflammatory responses, and within tumor microenvironments characterized by immune activation.

The LN3 antibody clone has become one of the most widely used reagents for detection of the HLA-DR antigen in immunology and pathology research. HLA-DR antibody LN3 recognizes the HLA-DR antigen complex associated with antigen-presenting immune cells and is commonly used to identify B lymphocytes, macrophages, dendritic cells, and other cells expressing MHC class II molecules in research applications. Detection of HLA-DR expression using antibodies such as clone LN3 supports studies of immune activation, antigen presentation pathways, and immune cell infiltration in both normal tissues and disease models.

Application Notes

Optimal dilution of the HLA-DR antibody clone LN3 should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Activated human peripheral blood mononuclear cells were used as the immunogen for the HLA-DR antibody.

Storage

Store the HLA-DR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

HLA-DR alpha antibody, HLA-DRA antibody, MHC class II DR antibody, HLA-DR antigen antibody