HLA-DRB1 Antibody for WB HLA-DRB/1067 / HLA-DRB1 Western Blot Antibody [clone HLA-DRB/1067] (V2590)

Description

Major histocompatibility complex class II DR beta 1 (HLA-DRB1) is a component of the HLA-DR antigen receptor encoded by the HLA-DRB1 gene and forms the beta chain of the major histocompatibility complex class II heterodimer responsible for antigen presentation to CD4-positive T lymphocytes. HLA-DRB1 Antibody for WB enables detection of the HLA-DR beta chain by western blot, supporting analysis of MHC class II expression in immune cell lysates and experimental models studying antigen presentation pathways.

The HLA-DR antigen receptor is composed of two transmembrane chains, an alpha chain encoded by HLA-DRA and a beta chain encoded by HLA-DRB genes. Together these chains assemble to form a heterodimeric receptor located on the cell surface of antigen-presenting cells. This receptor binds peptide fragments generated from extracellular proteins processed within endosomal compartments and presents them to CD4-positive helper T lymphocytes. Through this process the HLA-DR complex plays a central role in adaptive immune activation and immune surveillance.

HLA-DRB1 represents the principal beta chain gene of the HLA-DR complex and is highly polymorphic across human populations. Numerous HLA-DRB1 allelic variants contribute to immune recognition diversity and influence susceptibility to autoimmune diseases and infectious diseases. Because the beta chain participates directly in peptide binding and antigen presentation, detection of HLA-DRB1 expression is commonly used to evaluate antigen-presenting cell activity and immune signaling pathways in experimental systems.

Western blot analysis provides a useful approach for evaluating HLA-DRB1 protein expression in cell lysates and tissue extracts containing antigen-presenting immune cells. Antibodies directed against the HLA-DR beta chain enable researchers to confirm the presence of MHC class II molecules and study regulation of antigen presentation pathways in immune cell lines and biological samples. Mouse monoclonal clone HLA-DRB/1067 recognizes the HLA-DR beta chain and supports detection of HLA-DRB1 protein in western blot experiments designed to analyze MHC class II expression and immune-related signaling mechanisms.

Application Notes

Optimal dilution of the HLA-DRB1 Antibody for WB HLA-DRB/1067 should be determined by the researcher.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Activated human peripheral blood mononuclear cells were used as the immunogen for the HLA-DRB1 antibody.

Storage

Store the HLA-DRB1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

HLA-DR beta antibody, HLA-DRB1 antibody, MHC class II DR beta antibody, HLA class II histocompatibility antigen DR beta antibody