- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
HLA-DRB1 Antibody for IHC MSVA-478R / HLA-DRB1 Immunohistochemistry Antibody [clone MSVA-478R] (V6085)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V6085-100UG | Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide | 100 ug | 654.50 | |
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V6085-20UG | Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide | 20 ug | 324.50 |
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| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | MSVA-478R |
| UniProt | P01911 |
| Localization | Cell membrane |
| Applications | Immunohistochemistry (FFPE) : 1:100-1:200 |
| Limitations | This HLA-DRB1/Major histocompatibility complex class II DR beta 1 chain antibody is available for research use only. |
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Major histocompatibility complex class II DR beta 1 (HLA-DRB1) is a transmembrane glycoprotein encoded by the HLA-DRB1 gene that forms the beta chain of the HLA-DR antigen receptor complex. HLA-DRB1 Antibody for IHC is used to visualize the distribution of this antigen-presenting molecule within formalin-fixed, paraffin-embedded tissues by immunohistochemistry, enabling evaluation of immune cell populations and tissue immune activity directly within histologic sections.
The HLA-DR complex is a heterodimer composed of an invariant alpha chain encoded by HLA-DRA and a polymorphic beta chain encoded primarily by HLA-DRB1. Together these proteins assemble into a cell surface receptor responsible for presenting processed peptide antigens to CD4-positive helper T lymphocytes. This antigen presentation pathway is central to adaptive immune activation, coordinating interactions between antigen-presenting cells and T lymphocytes. As a result, expression of HLA-DR molecules is characteristic of professional antigen-presenting cells including B lymphocytes, macrophages, dendritic cells, and activated monocytes.
Immunohistochemistry analysis of HLA-DRB1 provides valuable insight into immune cell distribution within tissues. In lymphoid organs such as tonsil, lymph node, and spleen, strong membranous staining is typically observed in B-cell zones, interfollicular antigen-presenting cells, and dendritic cell populations. Macrophages and other antigen-presenting cells within inflammatory infiltrates may also demonstrate prominent HLA-DR expression. This staining pattern allows pathologists and researchers to identify immune cell populations and evaluate immune responses within normal tissues, inflammatory lesions, and tumor microenvironments.
HLA-DRB1 is highly polymorphic, with numerous allelic variants contributing to diversity in peptide binding and immune recognition across human populations. Variation in HLA-DRB1 has been associated with susceptibility to autoimmune diseases, transplant compatibility, and immune response variability. Because of these biological roles, antibodies targeting HLA-DRB1 are widely used in tissue-based immunohistochemistry studies examining immune infiltration, antigen-presenting cell localization, and immune activation in research and diagnostic settings.
A recombinant rabbit monoclonal antibody such as clone MSVA-478R enables consistent immunohistochemical detection of HLA-DR beta chain expression in tissue sections. The resulting staining typically highlights membranous and cytoplasmic patterns in antigen-presenting immune cells within lymphoid tissues and immune infiltrates, supporting morphological assessment of immune cell distribution in histologic specimens.
1. Optimal dilution of the HLA-DRB1 Antibody for IHC MSVA-478R should be determined by the researcher.
2. This HLA-DRB1/Major histocompatibility complex class II DR beta 1 chain antibody is recombinantly produced by expression in CHO cells.
3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Recombinant full-length human HLA-DRB1 protein was used as the immunogen for the HLA-DRB1/Major histocompatibility complex class II DR beta 1 chain antibody.
HLA-DRB1/Major histocompatibility complex class II DR beta 1 chain antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.
HLA-DR beta antibody, HLA-DRB1 antibody, MHC class II DR beta antibody, HLA class II histocompatibility antigen DR beta antibody
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