- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
HLA-DRA Antibody Recombinant Rabbit MAb for IHC / HLA-DR alpha immunohistochemistry antibody [clone MSVA-470R] (V6084)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V6084-100UG | Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide | 100 ug | 654.50 | |
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V6084-20UG | Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide | 20 ug | 324.50 |
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| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | MSVA-470R |
| UniProt | P01903 |
| Localization | Cell membrane |
| Applications | Immunohistochemistry (FFPE) : 1:100-1:200 |
| Limitations | This HLA-DRA/Major histocompatibility complex class II DR alpha chain antibody is available for research use only. |
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Human leukocyte antigen DR alpha (HLA-DRA) is a major histocompatibility complex class II protein encoded by the HLA-DRA gene and forms the alpha chain of the HLA-DR antigen complex involved in antigen presentation. This molecule pairs with the HLA-DR beta chain to form a heterodimeric receptor that presents processed peptide antigens to CD4-positive T lymphocytes, playing a critical role in adaptive immune responses. HLA-DRA is therefore a central component of the MHC class II antigen presentation pathway and is highly expressed in professional antigen-presenting cells including B lymphocytes, dendritic cells, macrophages, and certain activated epithelial and immune cells.
HLA-DRA Antibody Recombinant Rabbit MAb for IHC is used to detect expression of the HLA-DR alpha chain in formalin-fixed, paraffin-embedded tissues using immunohistochemistry. In normal tissues, HLA-DR alpha expression is typically observed in antigen-presenting cells within lymphoid organs and inflammatory microenvironments. Immunohistochemical staining often reveals membranous and cytoplasmic signal in B cells, macrophages, dendritic cells, and other immune cell populations involved in antigen processing and presentation. Detection of HLA-DRA by immunohistochemistry therefore provides valuable information about immune activation, antigen presentation, and inflammatory responses within tissue samples.
The HLA-DR antigen is widely used as a marker of immune activation and antigen-presenting cell identity in both normal and diseased tissues. Because the HLA-DR complex consists of alpha and beta chains encoded by separate genes, antibodies targeting the alpha chain such as HLA-DRA antibody reagents allow specific evaluation of this component of the MHC class II receptor. Expression of HLA-DR alpha is tightly regulated and can be induced by cytokines such as interferon-gamma during immune responses. As a result, HLA-DRA expression frequently increases in inflammatory conditions, infection, autoimmune disease, and tumor microenvironments where immune cell infiltration occurs.
In diagnostic and research pathology, HLA-DRA antibody staining is frequently used to evaluate immune cell populations and antigen presentation activity within tissues. The HLA-DR alpha chain is commonly detected in lymphoid tissues, sites of inflammation, and tumors with significant immune infiltration. Because HLA-DR expression reflects activation of antigen-presenting pathways, immunohistochemical analysis of HLA-DRA can help characterize immune microenvironments and assess interactions between immune cells and tumor cells. Clone MSVA-470R is a recombinant rabbit monoclonal antibody designed to recognize HLA-DRA and supports detection of the HLA-DR alpha chain in immunohistochemical staining applications.
1. Optimal dilution of the HLA-DRA antibody recombinant rabbit mAb for IHC should be determined by the researcher.
2. This HLA-DRA/Major histocompatibility complex class II DR alpha chain antibody is recombinantly produced by expression in human HEK293 cells.
3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
A recombinant fragment (around amino acids 1-200) of human HLA-DR protein (exact sequence is proprietary) was used as the immunogen for the HLA-DRA/Major histocompatibility complex class II DR alpha chain antibody.
HLA-DRA/Major histocompatibility complex class II DR alpha chain antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.
HLA-DR alpha antibody, HLA-DR antibody, MHC class II DR alpha antibody, HLA-DR antigen alpha chain antibody
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