- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
HLA-DRA Antibody for FACS 5B13F7 [clone 5B13F7] (RQ7131)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RQ7131 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 449 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Antigen affinity purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2b |
| Clone Name | 5B13F7 |
| Purity | Antigen affinity purified |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | P01903 |
| Applications | Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml Flow Cytometry : 1-3ug/million cells |
| Limitations | This HLA-DRA antibody is available for research use only. |
|
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Human leukocyte antigen DR alpha (HLA-DRA) is a major histocompatibility complex class II protein encoded by the HLA-DRA gene and forms the alpha chain of the HLA-DR antigen receptor responsible for antigen presentation to CD4-positive T lymphocytes. HLA-DRA Antibody for FACS enables detection of this immune marker on individual cells by flow cytometry, allowing identification and analysis of antigen-presenting cell populations such as B lymphocytes, macrophages, and dendritic cells.
HLA-DRA forms a heterodimer with the HLA-DR beta chain to create the HLA-DR antigen complex, a key component of the MHC class II antigen presentation pathway. This receptor binds peptides derived from extracellular proteins that have been processed within endosomal compartments and displays them at the cell surface for recognition by helper T cells. Through this mechanism, the HLA-DR alpha chain plays an essential role in immune surveillance and adaptive immune activation. Because antigen-presenting cells coordinate immune responses, HLA-DRA expression is commonly associated with professional antigen-presenting cells including dendritic cells, macrophages, and activated B cells.
HLA-DRA antibody reagents detect the alpha chain of the HLA-DR antigen, which is also referred to as HLA-DR alpha or the MHC class II DR alpha chain in immunology literature. Expression of HLA-DR alpha is tightly regulated by immune signaling pathways and can be strongly induced by inflammatory cytokines such as interferon-gamma. As a result, elevated HLA-DR expression is frequently observed in activated immune cells during infection, autoimmune responses, and inflammatory disease. Increased HLA-DR alpha expression can also occur in tumor microenvironments where immune infiltration and antigen presentation are active components of anti-tumor immunity.
Flow cytometry analysis of HLA-DRA expression provides a powerful approach for evaluating immune cell subsets and monitoring immune activation states within heterogeneous cell populations. Because flow cytometry measures fluorescence signals on a cell-by-cell basis, HLA-DRA antibodies are widely used to characterize antigen-presenting cells, assess immune activation, and study immune regulation in experimental systems. Detection of the HLA-DR alpha chain by flow cytometry is particularly valuable when analyzing blood leukocytes, cultured immune cells, or lymphoma-derived cell lines that naturally express high levels of MHC class II molecules. Clone 5B13F7 is a mouse monoclonal antibody that recognizes HLA-DRA and supports detection of the HLA-DR alpha chain in flow cytometry experiments designed to analyze immune cell populations and antigen presentation pathways.
Optimal dilution of the HLA-DRA antibody should be determined by the researcher.
Recombinant human protein (amino acids I26-L254) was used as the immunogen for the HLA-DRA antibody.
After reconstitution, the HLA-DRA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
HLA-DR alpha antibody, HLA-DR antibody, MHC class II DR alpha antibody, HLA-DR antigen alpha chain antibody
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