HLA-DR Antibody TAL 1B5 | HLA-DR Alpha Chain

	Catalog No	Formulation	Size	Price (USD)
	V7421-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	559
Formulation
	V7421-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	259
Formulation
	V7421SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	559
Formulation
	V7421IHC-7ML	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only	7 ml	559
Formulation

HLA-DR Antibody TAL 1B5 in Human Raji B Lymphoma Cells FACS. Flow cytometry analysis of human Raji B lymphoma cells with HLA-DR Antibody clone TAL 1B5. The antibody-stained population (blue) shows a strong rightward fluorescence shift compared with the isotype control (red), demonstrating robust detection of HLA-DR in Raji cells.

HLA-DR Antibody TAL 1B5 in Human Prostate Cancer IHC. Immunohistochemistry staining of FFPE human prostate cancer tissue with HLA-DR Antibody clone TAL 1B5. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM citrate buffer, pH 6, for 10-20 min followed by cooling before testing. Strong HLA-DR immunoreactivity is observed in the malignant glandular epithelium, with prominent membrane-associated and cytoplasmic staining.

HLA-DR Antibody TAL 1B5 in Human Lung Cancer IHC. Immunohistochemistry staining of FFPE human lung cancer tissue with HLA-DR Antibody clone TAL 1B5. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM citrate buffer, pH 6, for 10-20 min followed by cooling before testing. Strong HLA-DR immunoreactivity is observed in numerous tumor-associated cells, with prominent membrane-associated staining.

Purified HLA-DR Antibody TAL 1B5 SDS-PAGE. Electrophoretic analysis of 2 ug of purified, BSA-free HLA-DR Antibody clone TAL 1B5 under reducing (R) and non-reducing (NR) conditions. Under reducing conditions, bands are detected at approximately 50 kDa and 25 kDa, corresponding to the immunoglobulin heavy and light chains, respectively. Under non-reducing conditions, the intact antibody migrates at approximately 150 kDa, supporting the integrity and purity of the purified TAL 1B5 monoclonal antibody.

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HLA-DR Antibody TAL 1B5 in Human Raji B Lymphoma Cells FACS. Flow cytometry analysis of human Raji B lymphoma cells with HLA-DR Antibody clone TAL 1B5. The antibody-stained population (blue) shows a strong rightward fluorescence shift compared with the isotype control (red), demonstrating robust detection of HLA-DR in Raji cells.

HLA-DR Antibody TAL 1B5 in Human Prostate Cancer IHC. Immunohistochemistry staining of FFPE human prostate cancer tissue with HLA-DR Antibody clone TAL 1B5. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM citrate buffer, pH 6, for 10-20 min followed by cooling before testing. Strong HLA-DR immunoreactivity is observed in the malignant glandular epithelium, with prominent membrane-associated and cytoplasmic staining.

HLA-DR Antibody TAL 1B5 in Human Lung Cancer IHC. Immunohistochemistry staining of FFPE human lung cancer tissue with HLA-DR Antibody clone TAL 1B5. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM citrate buffer, pH 6, for 10-20 min followed by cooling before testing. Strong HLA-DR immunoreactivity is observed in numerous tumor-associated cells, with prominent membrane-associated staining.

Purified HLA-DR Antibody TAL 1B5 SDS-PAGE. Electrophoretic analysis of 2 ug of purified, BSA-free HLA-DR Antibody clone TAL 1B5 under reducing (R) and non-reducing (NR) conditions. Under reducing conditions, bands are detected at approximately 50 kDa and 25 kDa, corresponding to the immunoglobulin heavy and light chains, respectively. Under non-reducing conditions, the intact antibody migrates at approximately 150 kDa, supporting the integrity and purity of the purified TAL 1B5 monoclonal antibody.

Availability	1-3 business days
Species Reactivity	Human
Format	Purified
Host	Mouse
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG1, kappa
Clone Name	TAL 1B5
Purity	Protein G affinity chromatography
UniProt	P01903
Localization	Cell surface
Applications	Flow Cytometry : 1-2ug/10^6 cells Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations	This HLA-DR Antibody TAL 1B5 / HLA-DR Alpha Chain Antibody is available for research use only.
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Description

HLA-DR Antibody TAL 1B5 / HLA-DR Alpha Chain Antibody recognizes HLA-DR, a major histocompatibility complex (MHC) class II molecule central to antigen presentation and adaptive immunity. HLA-DR consists of an alpha chain encoded by HLA-DRA associated with a beta chain encoded by an HLA-DRB gene. Together, the two chains form a heterodimer that binds antigen-derived peptides for presentation to CD4-positive T cells, helping coordinate antigen-specific immune responses.

HLA-DR is prominently expressed by professional antigen-presenting cells, including B cells, monocytes, macrophages and dendritic cells, and expression can be induced in additional cell populations during immune activation. HLA-DR is consequently studied as both an antigen-presentation molecule and an immune-cell marker. Changes in HLA-DR expression are relevant to research involving inflammation, infectious disease, autoimmunity, transplantation and cancer, where MHC class II expression can influence interactions between antigen-presenting cells and helper T cells.

Clone TAL 1B5 is a well-established and extensively published monoclonal antibody with unusually detailed epitope characterization. Biochemical studies established that TAL 1B5 recognizes the HLA-DR alpha chain, and subsequent epitope-mapping experiments localized its binding site to the intracellular C-terminal tail of HLA-DRA. The antibody reacted with a synthetic peptide representing this region, while deletion of the C-terminal sequence eliminated TAL 1B5 recognition in Western blot and intracellular flow cytometry experiments. This defined molecular specificity provides particularly useful context for interpreting TAL 1B5 staining and distinguishes the clone from antibodies directed against extracellular or conformational HLA-DR determinants.

NSJ Bioreagents provides clone TAL 1B5 as an HLA-DR Antibody for research into HLA-DR expression, antigen presentation and immune-cell biology. Its extensive use in published research, together with its experimentally mapped HLA-DRA epitope, makes TAL 1B5 a particularly well-characterized reagent within the HLA-DR antibody family. An HLA-DR Alpha Chain Antibody can support investigation of MHC class II expression and mechanisms connecting HLA-DR biology with adaptive immune responses.

For additional information on HLA-DR biology and complementary antibody validation, visit our HLA-DR Antibody LN3 / MHC Class II Antibody page.

Application Notes

Optimal dilution of the HLA-DR Antibody TAL 1B5 / HLA-DR Alpha Chain Antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Human Bristol 8 cells were used as the immunogen for the HLA-DR Antibody TAL 1B5.

Storage

Store the HLA-DR Antibody TAL 1B5 at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

HLA-DR antibody, TAL 1B5 antibody, HLA-DRA antibody, HLA-DR Alpha Chain antibody, MHC Class II antibody

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