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Home >> Antibodies >> HLA-DQA1 Antibody / HLA-DQ Alpha 1 Chain Antibody

HLA-DQA1 Antibody / HLA-DQ Alpha 1 Chain Antibody (F54763)

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  Catalog No Formulation Size Price (USD)  
Image F54763-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 439
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F54763-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 205
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HLA-DQA1 Antibody in Human Daudi and Raji B Lymphoma Cells WB. Western blot testing of HLA-DQA1 Antibody / HLA-DQ Alpha 1 Chain Antibody with human 1) Daudi and 2) Raji B lymphoma cell lysate. A strong, discrete band is detected at approximately 36 kDa in both samples, consistent with the expected molecular weight of HLA-DQA1.
HLA-DQA1 Antibody in Human Spleen WB. Western blot testing of HLA-DQA1 Antibody / HLA-DQ Alpha 1 Chain Antibody with human spleen tissue lysate. A prominent band is detected at approximately 33-34 kDa, consistent with the expected molecular weight of HLA-DQA1.
HLA-DQA1 Antibody in Mouse NIH 3T3 Fibroblast Cells IF. Immunofluorescence staining of fixed and permeabilized mouse NIH 3T3 fibroblast cells with HLA-DQA1 Antibody / HLA-DQ Alpha 1 Chain Antibody (green) and DAPI nuclear stain (blue). Strong immunoreactivity is distributed throughout the cytoplasm with a granular and reticular appearance and relative exclusion from the nuclei.
HLA-DQA1 Antibody in Human K562 Myelogenous Leukemia Cells FACS. Flow cytometry testing of fixed and permeabilized human K562 myelogenous leukemia cells with HLA-DQA1 Antibody / HLA-DQ Alpha 1 Chain Antibody. The antibody-stained population (red) shows a clear fluorescence shift relative to the isotype control (blue), demonstrating detection of HLA-DQA1.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt P01909
Applications Western Blot : 1:2000-1:8000
Flow Cytometry : 1:25 (1x10e6 cells)
Immunofluorescence : 1:25
Limitations This HLA-DQA1 Antibody / HLA-DQ Alpha 1 Chain Antibody is available for research use only.
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Description

HLA-DQA1 Antibody / HLA-DQ Alpha 1 Chain Antibody targets HLA-DQA1, a major histocompatibility complex (MHC) class II protein that forms the alpha chain of the HLA-DQ heterodimer. HLA-DQ participates in adaptive immune recognition by presenting processed antigenic peptides to CD4-positive T lymphocytes. The HLA-DQA1 alpha chain associates non-covalently with an HLA-DQB beta chain to produce a functional cell-surface HLA-DQ molecule capable of binding and displaying peptides for T cell surveillance.

HLA-DQA1 is located within the MHC region on chromosome 6 and is highly polymorphic. The extracellular domains of the HLA-DQ alpha and beta chains together form the peptide-binding groove, with sequence variation contributing to differences in the repertoire of peptides that can be presented. HLA-DQA1 alleles combine with HLA-DQB1 alleles to generate diverse HLA-DQ heterodimers, and particular alpha/beta combinations are strongly associated with susceptibility or resistance to several immune-mediated diseases.

HLA-DQ molecules are expressed predominantly by professional antigen-presenting cells such as B lymphocytes, dendritic cells and macrophages. Proteins entering the endosomal pathway are processed into peptides that can be loaded onto MHC class II molecules following regulated removal of the invariant-chain-derived CLIP peptide. Peptide-loaded HLA-DQ complexes are subsequently transported to the plasma membrane, where they can interact with T cell receptors and the CD4 co-receptor to initiate antigen-specific immune responses.

Because HLA-DQA1 encodes an individual structural component of the HLA-DQ complex, an HLA-DQA1 Antibody provides a more chain-focused approach than antibodies recognizing the assembled HLA-DQ antigen or shared MHC class II determinants. HLA-DQA1 is an important research target in immunogenetics, antigen presentation, transplantation and autoimmune disease. Its extensive polymorphism and pairing with HLA-DQB proteins are particularly relevant to studies examining how inherited HLA variation influences peptide presentation and CD4 T cell recognition.

This antigen affinity-purified rabbit polyclonal antibody was generated against recombinant human protein. NSJ Bioreagents provides this HLA-DQA1 Antibody for research into HLA-DQ biology, MHC class II antigen presentation and adaptive immunity. An HLA-DQ Alpha 1 Chain Antibody can support investigation of the alpha-chain component of HLA-DQ and its role in antigen-specific immune recognition.

Explore our Immunology Antibodies page for additional reagents targeting MHC molecules, antigen presentation and adaptive immune responses.

Application Notes

The stated application concentrations are suggested starting points. Titration of the HLA-DQA1 Antibody / HLA-DQ Alpha 1 Chain Antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Recombinant human protein was used as the immunogen for the HLA-DQA1 antibody.

Storage

Aliquot the HLA-DQA1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Alternate Names

HLA-DQA1 antibody, HLA-DQ Alpha 1 Chain antibody, HLA-DQ Alpha Chain antibody, MHC Class II DQ Alpha antibody, DQA1 antibody

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