HLA-ABC Antibody MHC-I/8147R | MHC Class I Antibody

	Catalog No	Formulation	Size	Price (USD)
	V5128-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide	100 ug	559
Formulation
	V5128-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide	20 ug	259
Formulation
	V5128SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	559
Formulation

HLA-ABC Antibody MHC-I/8147R in Human Tonsil with Negative Control IHC. Immunohistochemistry testing of clone MHC-I/8147R in FFPE human tonsil tissue. HIER was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling before testing. Strong HLA-ABC immunoreactivity is observed throughout the tissue, with prominent cell-surface membrane staining. The inset shows the secondary antibody negative control, with PBS substituted for the primary antibody and minimal staining observed.

HLA-ABC Antibody MHC-I/8147R Membrane Staining in Human Tonsil IHC. Immunohistochemistry testing of clone MHC-I/8147R in FFPE human tonsil tissue following HIER by boiling in pH 9 10 mM Tris with 1 mM EDTA for 20 min and cooling before staining. HLA-ABC expression is broadly detected across the lymphoid tissue, with distinct membranous immunoreactivity outlining individual cells and highlighting the expected cell-surface distribution of MHC class I molecules.

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HLA-ABC Antibody MHC-I/8147R in Human Tonsil with Negative Control IHC. Immunohistochemistry testing of clone MHC-I/8147R in FFPE human tonsil tissue. HIER was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling before testing. Strong HLA-ABC immunoreactivity is observed throughout the tissue, with prominent cell-surface membrane staining. The inset shows the secondary antibody negative control, with PBS substituted for the primary antibody and minimal staining observed.

HLA-ABC Antibody MHC-I/8147R Membrane Staining in Human Tonsil IHC. Immunohistochemistry testing of clone MHC-I/8147R in FFPE human tonsil tissue following HIER by boiling in pH 9 10 mM Tris with 1 mM EDTA for 20 min and cooling before staining. HLA-ABC expression is broadly detected across the lymphoid tissue, with distinct membranous immunoreactivity outlining individual cells and highlighting the expected cell-surface distribution of MHC class I molecules.

Availability	1-3 business days
Species Reactivity	Human
Format	Purified
Host	Rabbit
Clonality	Recombinant Rabbit Monoclonal
Isotype	Rabbit IgG, kappa
Clone Name	MHC-I/8147R
Purity	Protein A/G affinity
UniProt	P01889 , P30443 , P30499
Localization	Cell Surface
Applications	Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations	This HLA-ABC Antibody MHC-I/8147R / MHC Class I Antibody is available for research use only.
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Description

HLA-ABC Antibody MHC-I/8147R / MHC Class I Antibody recognizes classical human major histocompatibility complex class I molecules represented by HLA-A, HLA-B and HLA-C. These highly polymorphic cell-surface glycoproteins are central components of adaptive immune surveillance. HLA class I molecules are expressed on nearly all nucleated cells and present intracellularly derived peptides to CD8-positive T lymphocytes, allowing the immune system to monitor cellular protein expression and identify cells affected by infection, malignant transformation or other intracellular abnormalities.

Classical MHC class I molecules consist of a polymorphic transmembrane heavy chain non-covalently associated with beta-2 microglobulin. The heavy chain contains alpha-1, alpha-2 and alpha-3 extracellular domains. The alpha-1 and alpha-2 domains form the peptide-binding groove, while beta-2 microglobulin contributes to structural stability of the assembled complex. HLA-A, HLA-B and HLA-C heavy chains are approximately 43-45 kDa glycoproteins, and their extensive genetic polymorphism enables recognition and presentation of a diverse repertoire of peptides.

Peptides presented by MHC class I molecules are generally generated through degradation of intracellular proteins. These peptides are transported into the endoplasmic reticulum by the transporter associated with antigen processing (TAP), where they are loaded onto newly assembled HLA class I molecules. Stable complexes containing the HLA heavy chain, beta-2 microglobulin and bound peptide subsequently pass through the Golgi apparatus to the plasma membrane. At the cell surface, peptide-HLA complexes can be surveyed by CD8-positive cytotoxic T cells.

HLA class I biology is important in research involving viral immunity, tumor immunology, transplantation, autoimmunity and immune evasion. Changes in HLA-A, HLA-B or HLA-C expression can influence antigen recognition by cytotoxic T cells, while class I molecules also participate in interactions with receptors expressed by natural killer cells. Consequently, altered MHC class I expression or antigen presentation can affect the balance between immune recognition and immune escape.

Clone MHC-I/8147R is a recombinant rabbit monoclonal antibody generated against a recombinant partial sequence within amino acids 100-300 of human HLA class I protein. NSJ Bioreagents provides this HLA-ABC Antibody for research into HLA class I biology, antigen presentation and immune surveillance. An MHC Class I Antibody can support investigation of the mechanisms connecting intracellular antigen processing with T cell-mediated immune recognition.

Explore our Immunology Antibodies page for additional reagents targeting proteins involved in antigen presentation, immune recognition and adaptive immune responses.

Application Notes

Optimal dilution of the HLA-ABC Antibody MHC-I/8147R / MHC Class I Antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 100-300) from the human protein was used as the immunogen for the HLA-ABC antibody.

Storage

Aliquot the HLA-ABC antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Alternate Names

HLA-ABC antibody, MHC Class I antibody, MHC I antibody, HLA Class I antibody, HLA-A antibody, HLA-B antibody, HLA-C antibody

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HLA-ABC Antibody MHC-I/8147R / MHC Class I Antibody [clone MHC-I/8147R] (V5128)

Description

Application Notes

Immunogen

Storage

Alternate Names

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Area of Research

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Protocols

Contact Us

NSJ Bioreagents