HK1 Antibody 4B7 / Glycolysis Enzyme Antibody [clone 4B7] (RQ5660)

HK1 Antibody 4B7 in Human Lung Cancer IF. Immunofluorescence testing of clone 4B7 in FFPE human lung cancer tissue. HIER was performed by steaming tissue sections in pH 6 citrate buffer for 20 min. HK1 immunoreactivity (green) is observed predominantly in the cytoplasm of epithelial cells, with DAPI nuclear counterstaining shown in blue.

HK1 Antibody 4B7 in Human U-2 OS Osteosarcoma Cells IF. Immunofluorescence testing of clone 4B7 in FFPE human U-2 OS osteosarcoma cells. HIER was performed by steaming in pH 6 citrate buffer for 20 min. HK1 immunoreactivity (green) is observed predominantly in the cytoplasm, with a punctate to granular distribution consistent with the mitochondrial-associated localization of this glycolysis enzyme. Nuclei are counterstained with DAPI (blue).

Immunohistochemistry testing of clone 4B7 in FFPE human breast cancer tissue. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. Strong HK1 immunoreactivity is observed predominantly in the cytoplasm of glandular tumor cells.

HK1 Antibody 4B7 in Human Lung Cancer IHC. Immunohistochemistry testing of clone 4B7 in FFPE human lung cancer tissue. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. Strong HK1 immunoreactivity is observed predominantly in the cytoplasm and along the cell periphery of tumor cells.

HK1 Antibody 4B7 in Human Testis Cancer IHC. Immunohistochemistry testing of clone 4B7 in FFPE human testis cancer tissue. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. HK1 immunoreactivity is observed predominantly in the cytoplasm of cells within the tumor tissue.

HK1 Antibody 4B7 in Mouse Intestine IHC. Immunohistochemistry testing of clone 4B7 in FFPE mouse intestine tissue. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. Strong HK1 immunoreactivity is observed in intestinal epithelial cells, with prominent cytoplasmic staining along the villous epithelium.

HK1 Antibody 4B7 in Rat Intestine IHC. Immunohistochemistry testing of clone 4B7 in FFPE rat intestine tissue. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. Strong HK1 immunoreactivity is observed predominantly in the cytoplasm of intestinal epithelial cells.

HK1 Antibody 4B7 in Human, Rat and Mouse Cell Lysates WB. Western blot testing of clone 4B7 in 1) human HeLa, 2) human HEK293, 3) human U-87 MG, 4) human K562, 5) human A549, 6) rat PC-12 and 7) mouse NIH 3T3 cell lysates. A strong, discrete HK1 band is observed at approximately 100-110 kDa across all seven samples. The observed migration is consistent with full-length hexokinase-1 and demonstrates detection in human, rat and mouse lysates.

HK1 Antibody 4B7 in Human PC-3 Prostate Cancer Cells FACS. Flow cytometry testing of clone 4B7 in human PC-3 prostate cancer cells at 1 ug/million cells following blocking with goat sera. The HK1 antibody-stained population (blue) shows a strong fluorescence shift relative to the isotype control (green) and cells-alone control (red), demonstrating detection of HK1 under the tested conditions.