- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
HJURP Antibody / Holliday junction recognition protein (FY12913)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
FY12913 | Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml | 100 ug | 439 |
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HJURP antibody detects Holliday junction recognition protein, a histone chaperone responsible for depositing the centromere-specific histone H3 variant CENP-A at centromeres during DNA replication. Encoded by the HJURP gene on chromosome 2q37.1, this protein is essential for centromere identity and chromosome segregation fidelity. HJURP functions as a dedicated assembly factor that ensures proper incorporation of newly synthesized CENP-A into centromeric nucleosomes during the cell cycle.
Structurally, HJURP is a 748-amino-acid nuclear protein of approximately 80 kilodaltons containing an N-terminal Scm3-like domain that binds CENP-A/H4 heterodimers and a C-terminal domain responsible for centromere targeting through interaction with Mis18 complex components. HJURP localizes exclusively to centromeres during late telophase and early G1 phase, coordinating with the Mis18alpha/beta complex to mediate chromatin assembly and maintain centromere identity.
The HJURP antibody is widely used in cell cycle, chromatin assembly, and cancer research to study centromere formation, histone variant deposition, and chromosomal stability. Western blot analysis detects an 80 kilodalton band corresponding to HJURP, while immunofluorescence reveals discrete nuclear foci at centromeric regions. This antibody provides a key tool for analyzing chromosome segregation fidelity and CENP-A deposition dynamics.
Functionally, HJURP acts as the exclusive chaperone for CENP-A nucleosome assembly, ensuring the maintenance of centromeric chromatin identity across cell divisions. Loss or depletion of HJURP leads to chromosome mis-segregation, aneuploidy, and genomic instability, hallmark features of tumorigenesis. Elevated HJURP expression has been reported in various cancers and correlates with poor prognosis due to its role in promoting proliferation and mitotic progression. The HJURP antibody supports studies on chromatin inheritance, mitotic regulation, and epigenetic maintenance of centromeres. NSJ Bioreagents validates this antibody for western blotting, immunohistochemistry, and immunofluorescence, providing a dependable reagent for centromere biology and mitotic research.
Optimal dilution of the HJURP antibody should be determined by the researcher.
E.coli-derived human HJURP recombinant protein (Position: R6-K594) was used as the immunogen for the HJURP antibody.
After reconstitution, the HJURP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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