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Home >> Antibodies >> Hjurp Antibody / Fleg1

Hjurp Antibody / Fleg1 (RQ6561)

  Catalog No Formulation Size Price (USD)  
Image RQ6561 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of mouse ANA-1 cells with Hjurp antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Hjurp antibody.
Western blot testing of 1) mouse stomach, 2) mouse liver, 3) mouse kidney, 4) mouse RAW264.7, 5) rat stomach, 6) rat liver and 7) rat kidney lysate with Hjurp antibody. Expected molecular weight: 74-90 kDa.
Availability 1-3 business days
Species Reactivity Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q6PG16
Applications Western blot : 1-2ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Hjurp antibody is available for research use only.
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Description

Holliday junction recognition protein is a protein in humans that is encoded by the HJURP gene. HJURP (holliday junction recognition protein) is a protein coding gene. Diseases associated with HJURP include fibrillary astrocytoma. Among its related pathways are DNA damage and chromosome maintenance. Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. Directly binds holliday junctions.

Application Notes

Optimal dilution of the Hjurp antibody should be determined by the researcher.

Immunogen

An E. coli-derived mouse protein (amino acids Q13-T574) was used as the immunogen for the Hjurp antibody.

Storage

After reconstitution, the Hjurp antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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