Histone H4 Antibody / Multi Gene HIST1H4 Family Chromatin Antibody (FY12427)

Histone H4 Antibody for IF. Immunofluorescence analysis of multi-gene histone H4 expression in human U2OS cells using Histone H4 Antibody (green). Strong nuclear staining is observed, consistent with localization of HIST1H4 family proteins within chromatin across multiple histone H4 gene variants. Beta Tubulin (red) highlights the cytoskeletal network, providing cellular context and contrast to the nuclear histone signal. Nuclei are visualized with DAPI (blue), confirming uniform nuclear distribution of histone H4 as a core nucleosomal protein.

Histone H4 Antibody for WB. Western blot analysis of multi-gene histone H4 expression in cell and tissue lysates. Lane 1: human HeLa cells, Lane 2: human 293T cells, Lane 3: human HepG2 cells, Lane 4: human HT1080 cells, Lane 5: rat kidney tissue, Lane 6: rat thymus tissue, Lane 7: mouse kidney tissue, Lane 8: mouse thymus tissue. A band is detected at approximately 14 kDa, compared to the predicted molecular weight of Histone H4 (HIST1H4) at approximately 11 kDa. The slightly higher apparent molecular weight is consistent with histone post-translational modifications such as acetylation or phosphorylation, which can alter electrophoretic mobility. Consistent detection across human, rat, and mouse samples reflects broad recognition of HIST1H4 family proteins and conserved chromatin-associated expression.

Immunofluorescent staining of Histone H4 using anti-Histone H4 antibody. Histone H4 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/ml rabbit anti-Histone H4 antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue). Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Histone H4 Antibody for IHC. Immunohistochemistry analysis of multi-gene histone H4 expression in FFPE human breast cancer tissue using Histone H4 Antibody. Nuclear HRP-DAB brown staining is observed in tumor epithelial cells, consistent with localization of HIST1H4 family proteins within chromatin across multiple histone H4 gene variants. The staining highlights strong nuclear compartmentalization with minimal cytoplasmic signal, reflecting the role of histone H4 as a core nucleosomal protein in chromatin structure.

Flow Cytometry analysis of 293T cells using anti-Histone H4 antibody. Overlay histogram showing 293T cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Histone H4 antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Flow Cytometry analysis of C6 cells using anti-Histone H4 antibody. Overlay histogram showing C6 cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Histone H4 antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Flow Cytometry analysis of HEPA1-6 cells using anti-Histone H4 antibody. Overlay histogram showing HEPA1-6 cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Histone H4 antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.