- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Histone H3 (mono methyl K79) Antibody / HIST1H3A Chromatin Organization Antibody [clone 31H92] (FY13214)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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FY13214 | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA | 100 ul | 449 |
| Availability | 2-3 weeks |
| Species Reactivity | Human, Mouse, Rat |
| Format | Liquid |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Clone Name | 31H92 |
| Purity | Affinity chromatography |
| Buffer | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. |
| UniProt | P68431 |
| Applications | Western Blot : 1:500-1:2000 Immunohistochemistry : 1:50-1:200 Immunocytochemistry/Immunofluorescence : 1:50-1:200 |
| Limitations | This Histone H3 (mono methyl K79) antibody is available for research use only. |
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Histone H3 (HIST1H3A) contains internal lysine residues whose methylation contributes to chromatin architecture and nucleosome organization. Monomethylation at lysine 79 is a unique modification because it occurs within the histone core domain rather than the exposed N-terminal tail. Histone H3 (mono methyl K79) Antibody / HIST1H3A Chromatin Organization Antibody (clone 31H92) is designed to detect this site-specific methylation state as a marker of intranucleosomal chromatin regulation. This antibody is part of a broader collection of Histone H3 antibodies used to study chromatin structure, histone modifications, and epigenetic regulation.
HIST1H3A antibody, also referred to as Histone H3 antibody and H3K79me1 antibody in the literature, recognizes a modification associated with chromatin organization within actively regulated genomic regions. The location of Lys79 within the nucleosome core enables it to influence chromatin structure in a way that is fundamentally different from tail-based modifications such as H3K4me1, H3K9me1, or acetylated lysine residues.
This recombinant rabbit monoclonal clone 31H92 antibody is uniquely positioned for studies of chromatin organization and nucleosomal methylation architecture rather than transcriptional activation alone. While other pages emphasize transcription-linked gene body methylation, this differentiation focuses on the structural role of core-domain methylation in maintaining chromatin integrity and nucleosome interactions.
At the molecular level, H3K79 monomethylation is catalyzed by DOT1L and contributes to nucleosome stability, chromatin organization, and regulated transcriptional states. Its position within the histone fold domain suggests a role in shaping nucleosomal geometry and chromatin compaction rather than directly modulating transcription factor accessibility.
This modification provides insight into chromatin architecture within active genomic regions and may influence higher-order chromatin organization in addition to supporting transcription-associated processes. Its structural role distinguishes it from acetylation-based chromatin relaxation mechanisms.
In western blot applications, the antibody detects Histone H3 at approximately 15 kDa, with signal corresponding to monomethylated Histone H3 within chromatin. Detection reflects intranucleosomal methylation states and chromatin organization rather than enhancer activation, promoter accessibility, or replication-associated chromatin assembly.
At the cellular level, H3K79 monomethylation localizes to the nucleus and is associated with organized euchromatic regions involved in regulated gene expression. This distribution supports its use in studying chromatin structure and nucleosome-level epigenetic regulation.
This antibody supports detection of Lys79-monomethylated Histone H3, enabling investigation of nucleosome core methylation, chromatin organization, and epigenetic regulation of chromatin structure.
Optimal dilution of the Histone H3 (mono methyl K79) Antibody / HIST1H3A Chromatin Organization Antibody should be determined by the researcher.
A synthesized peptide derived from human Histone H3 (mono methyl K79) was used as the immunogen for the Histone H3 (mono methyl K79) Antibody / HIST1H3A Chromatin Organization Antibody.
Store the Histone H3 (mono methyl K79) antibody at -20oC.
Histone H3 Lys79 monomethylation antibody, H3K79me1 chromatin organization antibody, histone H3 mono methyl Lys79 antibody, intranucleosomal methylation antibody
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