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Home >> Antibodies >> H3K9ac Antibody / HIST1H3A Promoter Activation Marker Antibody

H3K9ac Antibody / HIST1H3A Promoter Activation Marker Antibody [clone 32H23] (FY13175)

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Image FY13175 Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA 100 ul 449
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H3K9ac Antibody / HIST1H3A Promoter Activation Marker Antibody (clone 32H23) for WB. Western blot analysis of HIST1H3A / Histone H3 Lys9 acetylation in human cell lysates including 293T (lane 1), U2OS (lane 2), T98G (lane 3), whole cell lysate (lane 4), K562 (lane 5), SH-SY5Y (lane 6), and U251 (lane 7) using H3K9ac Antibody / HIST1H3A Promoter Activation Marker Antibody. A band is detected at the predicted molecular weight of approximately 15 kDa corresponding to acetylated Histone H3, with consistent signal across multiple cell lines reflecting promoter-associated chromatin activation. GAPDH is shown as a loading control.
Immunohistochemical staining of Histone H3 using anti-Histone H3 (acetyl K9) antibody. Histone H3 (acetyl K9) was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Histone H3 (acetyl K9) antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
Immunohistochemical staining of Histone H3 (acetyl K9) using anti-Histone H3 (acetyl K9) antibody. Histone H3 (acetyl K9) was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Histone H3 (acetyl K9) antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
H3K9ac Antibody / HIST1H3A Promoter Activation Marker Antibody (clone 32H23). Immunohistochemistry analysis of HIST1H3A / Histone H3 Lys9 acetylation in rat brain tissue using H3K9ac Antibody / HIST1H3A Promoter Activation Marker Antibody. Strong HRP-DAB brown nuclear staining is observed in neuronal and glial cell populations, consistent with euchromatic localization and promoter-associated transcriptional activation, while background staining remains low in surrounding tissue structures.
H3K9ac Antibody / HIST1H3A Promoter Activation Marker Antibody (clone 32H23). Immunohistochemistry analysis of HIST1H3A / Histone H3 Lys9 acetylation in human breast tissue using H3K9ac Antibody / HIST1H3A Promoter Activation Marker Antibody. Strong HRP-DAB brown nuclear staining is observed in epithelial cells within breast tissue, consistent with euchromatic localization and promoter-associated transcriptional activation, while stromal cells show comparatively lower nuclear signal.
Western blot testing of mouse samples using the Histone H3 (acetyl K9) antibody at 1:2000 dilution for 1 hour at room temperature. The expected molecular weight of Histone H3 (acetyl K9) is at 15 kDa.
Western blot testing of rat samples using the Histone H3 (acetyl K9) antibody at 1:2000 dilution for 1 hour at room temperature. The expected molecular weight of Histone H3 (acetyl K9) is at 15 kDa.
H3K9ac Antibody / HIST1H3A Promoter Activation Marker Antibody (clone 32H23) for WB. Western blot analysis of HIST1H3A / Histone H3 Lys9 acetylation in trichostatin A-treated rat C6 cell lysate using H3K9ac Antibody / HIST1H3A Promoter Activation Marker Antibody. A band is detected at the predicted molecular weight of approximately 15 kDa corresponding to acetylated Histone H3, with signal consistent with increased lysine acetylation following histone deacetylase inhibition and enhanced promoter-associated chromatin activation.
Immunohistochemical staining of Histone H3 (acetyl K9) using anti-Histone H3 (acetyl K9) antibody. Histone H3 (acetyl K9) was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Histone H3 (acetyl K9) antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
Availability 2-3 weeks
Species Reactivity Human, Mouse, Rat
Format Liquid
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name 32H23
Purity Affinity chromatography
Buffer Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
UniProt P68431
Localization Nuclear
Applications Western Blot : 1:500-1:2000
Immunohistochemistry : 1:50-1:200
Immunocytochemistry/Immunofluorescence : 1:50-1:200
Immunoprecipitation : 1:50
Flow Cytometry : 1:50
Limitations This Histone H3 (acetyl K9) antibody is available for research use only.
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Description

Histone H3 (HIST1H3A) is a core nucleosomal protein that undergoes acetylation at specific lysine residues to regulate chromatin structure and gene expression. Acetylation at lysine 9 is one of the most well-characterized histone modifications associated with transcriptional activation at promoter regions. H3K9ac Antibody / HIST1H3A Promoter Activation Marker Antibody (clone 32H23) is designed to detect Histone H3 acetylated at lysine 9, providing a robust and widely accepted marker of transcription initiation and promoter activation. This antibody is part of a broader collection of Histone H3 antibodies used to study chromatin structure, histone modifications, and epigenetic regulation.

HIST1H3A antibody, also referred to as Histone H3 antibody and H3K9ac antibody in the literature, recognizes a modification catalyzed by histone acetyltransferases that neutralizes the positive charge of lysine residues. This reduces histone-DNA interactions and promotes local chromatin relaxation, allowing transcription factors and RNA polymerase complexes to access DNA. H3K9 acetylation is strongly enriched at transcription start sites and active promoter regions, making it a key indicator of genes undergoing active transcription initiation.

This recombinant rabbit monoclonal clone 32H23 antibody is uniquely positioned as a primary promoter activation marker within the histone acetylation landscape. Compared to enhancer-associated marks such as H3K27ac or elongation-associated marks such as H3K36ac, H3K9ac is most closely linked to promoter-proximal chromatin activation and early transcriptional engagement. This makes it particularly valuable for distinguishing promoter-driven transcriptional activity from downstream regulatory events.

At the molecular level, H3K9 acetylation facilitates recruitment of bromodomain-containing proteins, transcriptional co-activators, and chromatin remodeling complexes. These interactions support assembly of the transcriptional machinery and stabilization of open chromatin at promoter regions. The modification often co-occurs with other activating marks, but retains a distinct functional role in defining transcriptionally competent promoters.

In western blot applications, the antibody detects Histone H3 at approximately 15 kDa, with signal corresponding to acetylated chromatin associated with transcriptionally active promoters. Detection reflects promoter activation states rather than global chromatin accessibility or mitotic chromatin condensation.

At the cellular level, H3K9 acetylation localizes to the nucleus and is enriched in euchromatic regions associated with active gene expression. This distribution clearly distinguishes it from repressive methylation at lysine 9, highlighting its role in transcriptional activation rather than gene silencing.

This antibody supports detection of Lys9-acetylated Histone H3, enabling detailed investigation of promoter activation, transcription initiation, and chromatin accessibility in gene regulation studies.

Application Notes

Optimal dilution of the H3K9ac Antibody / HIST1H3A Promoter Activation Marker Antibody should be determined by the researcher.

Immunogen

A synthesized peptide derived from human Histone H3 (acetyl K9) was used as the immunogen for the H3K9ac Antibody / HIST1H3A Promoter Activation Marker Antibody.

Storage

Store the Histone H3 (acetyl K9) antibody at -20oC.

Alternate Names

Histone H3 Lys9 acetylation antibody, H3K9ac promoter antibody, histone H3 acetyl Lys9 antibody, transcription initiation histone antibody

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