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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Histone H2B (crotonyl K12) Antibody / HIST2H2BE [clone 31H73] (FY12726)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
FY12726 | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA | 100 ul | 439 |
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Histone H2B (crotonyl K12) antibody detects histone H2B specifically when modified by crotonylation at lysine 12. Histone H2B is encoded by multiple genes including HIST2H2BE, HIST1H2BB, and HIST1H2BC, with common identifiers such as H2B type 2E and H2B histone family members. Histone H2B is one of the four core histones that assemble into nucleosomes, which package and regulate DNA. Crotonylation is a recently discovered lysine acylation modification linked to transcriptional activation and open chromatin states. Modification at lysine 12 of H2B provides a dynamic marker of gene regulation in specific contexts such as development and stress responses.
Histone H2B (crotonyl K12) antibody is widely applied in epigenetics, chromatin biology, and cancer research. By detecting crotonylation at this specific residue, researchers can examine how histone modifications extend beyond acetylation and methylation to influence transcription. Crotonylation at K12 has been associated with gene activation in embryonic stem cells, immune regulation, and cellular stress adaptation.
The antibody is validated for chromatin immunoprecipitation, western blotting, and immunofluorescence. ChIP with Histone H2B (crotonyl K12) antibody identifies promoter and enhancer regions enriched in this mark. Western blotting distinguishes crotonylated H2B from unmodified protein, while immunofluorescence highlights nuclear distribution of modified nucleosomes. These applications provide robust methods for exploring the biological relevance of histone crotonylation.
Histone crotonylation is regulated by histone acetyltransferases and deacetylases, many of which also act on acetylation. This overlap suggests coordination between acylation states in regulating gene activity. Dysregulation of crotonylation has been implicated in cancer, neurodegeneration, and metabolic disease. Histone H2B (crotonyl K12) antibody supports studies into how this modification contributes to disease pathology and therapeutic opportunities.
Histone H2B (crotonyl K12) antibody from NSJ Bioreagents provides dependable specificity for this site specific modification. Its performance ensures accurate evaluation of epigenetic regulation across cellular and disease models.
Optimal dilution of the Histone H2B (crotonyl K12) antibody should be determined by the researcher.
A synthesized peptide derived from human Histone H2B (crotonyl K12) was used as the immunogen for the Histone H2B (crotonyl K12) antibody.
Store the Histone H2B (crotonyl K12) antibody at -20oC.
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