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Home >> Antibodies >> Histone H1 Antibody

Histone H1 Antibody (V3323)

  Catalog No Formulation Size Price (USD)  
Image V3323-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3323-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3323SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Immunofluorescent staining of PFA-fixed human HeLa cells with Histone H1 antibody (green) and Phalloidin (red).
IHC testing of FFPE human tonsil and Histone H1 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of permeabilized human HeLa cells with Histone H1 antibody; Red=isotype control, Blue= Histone H1 antibody.
Western blot testing of human heart lysate with Histone H1 antibody. Observed molecular weight ~22/30-33 kDa (unmodified/modified).
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Protein A affinity chromatography
UniProt P07305
Localization Nuclear
Applications Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Western blot : 1-2ug/ml
Limitations This Histone H1 antibody is available for research use only.
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Description

Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. [UniProt]

Application Notes

Optimal dilution of the Histone H1 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human protein was used as the immunogen for the Histone H1 antibody.

Storage

Store the Histone H1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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