Histone Deacetylase 1 Antibody / Chromatin Deacetylase Antibody (RQ7277)

Histone Deacetylase 1 Antibody in Human Tonsil IHC. Immunohistochemistry testing of Histone Deacetylase 1 Antibody in FFPE human tonsil tissue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling prior to staining. Nuclear immunoreactivity is observed throughout the lymphoid tissue, consistent with the chromatin-associated function of HDAC1.

Histone Deacetylase 1 Antibody in Human Lung Cancer IHC. Immunohistochemistry testing of Histone Deacetylase 1 Antibody in FFPE human lung cancer tissue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling prior to staining. Nuclear HDAC1 immunoreactivity is observed in tumor cells, consistent with its role in chromatin deacetylation and transcriptional regulation.

Histone Deacetylase 1 Antibody in Human Esophageal Squamous Carcinoma IHC. Immunohistochemistry testing of Histone Deacetylase 1 Antibody in FFPE human esophageal squamous carcinoma tissue. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling prior to staining. Prominent nuclear immunoreactivity is observed in tumor cells, consistent with the nuclear localization of HDAC1.

Histone Deacetylase 1 Antibody in Human Leukemia and Glioma Cells WB. Western blot testing of Histone Deacetylase 1 Antibody in human 1) MOLT-4 T-cell leukemia, 2) Jurkat T-cell leukemia, 3) HL-60 promyelocytic leukemia and 4) U-251 glioma cell lysates. A prominent band is observed at approximately 55-60 kDa across the tested samples, consistent with the predicted molecular weight of HDAC1. This Chromatin Deacetylase Antibody demonstrates detection of endogenous HDAC1 across multiple human cell lines.

Histone Deacetylase 1 Antibody in Human MCF-7 Breast Cancer Cells FACS. Flow cytometry testing of human MCF-7 breast cancer cells with Histone Deacetylase 1 Antibody at 1 ug/million cells following blocking with goat serum. Cells alone are shown in red, the isotype control in green and HDAC1 antibody staining in blue. A clear fluorescence shift is observed relative to both controls, demonstrating detection of the chromatin deacetylase HDAC1 in the tested cells.