Histone H1 Antibody / Total Linker Histone H1 Chromatin Antibody [clone AE-4] (V2565)

Description

Histone H1 is a linker histone that binds to nucleosome entry and exit sites and plays a central role in chromatin organization, DNA packaging, and higher-order chromatin folding. Histone H1 Antibody detects total linker histone H1 protein independent of variant or modification status, providing a comprehensive measure of H1 abundance within chromatin. Unlike core histones, histone H1 functions outside the nucleosome core and is essential for stabilizing linker DNA and organizing nucleosome arrays. This antibody is part of our broader Histone H1 antibody collection, including linker histone variants, chromatin organization, chromatin accessibility, and nuclear architecture reagents for chromatin and epigenetics research.

Histone H1 antibody, also referred to as H1 antibody or linker histone antibody in the literature, is widely used to evaluate chromatin structure and genome organization. The well-characterized clone AE-4 antibody is frequently used for detection of total histone H1 and has been utilized in numerous peer-reviewed studies, supporting its use in chromatin biology research. Clone AE-4 antibody enables robust detection of H1 across experimental systems, making it a reliable tool for assessing linker histone distribution and chromatin organization.

Functionally, histone H1 binds to linker DNA at the nucleosome entry and exit points, stabilizing DNA conformation and promoting interactions between adjacent nucleosomes. This facilitates folding of chromatin into higher-order structures and contributes to genome compaction. As a result, histone H1 is a key determinant of chromatin density and plays a major role in regulating DNA accessibility at a global level.

Histone H1 is expressed as multiple variants that are dynamically regulated during the cell cycle, differentiation, and development. Changes in H1 levels can influence chromatin condensation, nucleosome spacing, and overall genome organization. Because histone H1 is broadly distributed across chromatin, detection of total H1 provides insight into global chromatin structure rather than localized chromatin events.

In chromatin studies, total histone H1 detection complements analysis of core histones and histone modifications by providing information about linker DNA organization and higher-order chromatin folding. This allows researchers to evaluate chromatin structure at multiple levels, from nucleosome composition to large-scale chromatin organization.

A rabbit monoclonal antibody such as clone AE-4 enables reliable detection of total histone H1, supporting studies of chromatin structure, DNA packaging, and genome organization across diverse biological systems.

Application Notes

Optimal dilution of the Histone H1 Antibody / Total Linker Histone H1 Chromatin Antibody should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Nuclei of human leukemia biopsy cells were used as the immunogen for the Histone H1 Antibody / Total Linker Histone H1 Chromatin Antibody.

Storage

Store the Histone H1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Histone H1 antibody, H1 antibody, linker histone H1 antibody, total H1 antibody