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Home >> Antibodies >> Histone acetyltransferase 1 Antibody / HAT1 / KAT1

Histone acetyltransferase 1 Antibody / HAT1 / KAT1 (RQ6485)

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  Catalog No Formulation Size Price (USD)  
Image RQ6485 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human Caco-2 cells with Histone acetyltransferase 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human Caco-2 cells with Histone acetyltransferase 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) rat stomach, 2) rat testis and 3) mouse testis tissue lysate with Histone acetyltransferase 1 antibody. Predicted molecular weight: 50 kDa (isoform a).
Flow cytometry testing of human U-87 MG cells with Histone acetyltransferase 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Histone acetyltransferase 1 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt O14929
Localization Cytoplasmic, nuclear
Applications Western blot : 1-2ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This Histone acetyltransferase 1 antibody is available for research use only.
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Description

Histone acetyltransferase 1, also known as HAT1, is an enzyme that, in humans, is encoded by the HAT1 gene. The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene.

Application Notes

Optimal dilution of the Histone acetyltransferase 1 antibody should be determined by the researcher.

Immunogen

Amino acids EKFLVEYKSAVEKKLAEYK from the human protein were used as the immunogen for the Histone acetyltransferase 1 antibody.

Storage

After reconstitution, the Histone acetyltransferase 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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