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Home >> Antibodies >> HINT1 Antibody

HINT1 Antibody [clone 1500CT836.13.93] (F54529)

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  Catalog No Formulation Size Price (USD)  
Image F54529-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54529-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of human 1) Jurkat, 2) Raji, 3) HepG2, 4) rat brain and 5) mouse liver lysate with HINT1 antibody. Expected molecular weight ~14 kDa.
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Western blot testing of human 1) Jurkat, 2) Raji, 3) HepG2, 4) rat brain and 5) mouse liver lysate with HINT1 antibody. Expected molecular weight ~14 kDa.
IHC testing of FFPE human kidney tissue with HINT1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human U-2 OS cells with HINT1 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Flow cytometry testing of fixed and permeabilized human Jurkat cells with HINT1 antibody; Blue=isotype control, Green= HINT1 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype IgG1, kappa
Clone Name 1500CT836.13.93
Purity Protein G affinity
UniProt P49773
Localization Cytoplasmic, nuclear
Applications Western blot : 1:500-1:2000
Flow cytometry : 1:25 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:25
Immunofluorescence : 1:25
Limitations This HINT1 antibody is available for research use only.
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  • Applications : WB, IHC-P, IF, FACS
    Reactivity : Human, Mouse, Rat

Description

Hydrolyzes purine nucleotide phosphoramidates with a single phosphate group, including adenosine 5'monophosphoramidate (AMP-NH2), adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His- AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O- phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide. In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1. Modulates p53/TP53 levels and p53/TP53-mediated apoptosis. Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin- protein ligase complex.

Application Notes

The stated application concentrations are suggested starting points. Titration of the HINT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Recombinant human protein was used as the immunogen for the HINT1 antibody.

Storage

Aliquot the HINT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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