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Home >> Antibodies >> HIF3A Antibody / Hypoxia-inducible factor 3 alpha

HIF3A Antibody / Hypoxia-inducible factor 3 alpha (RQ5626)

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  Catalog No Formulation Size Price (USD)  
Image RQ5626 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat lung, 2) rat skeletal muscle, 3) mouse lung and 4) mouse NIH 3T3 lysate with HIF3A antibody. Expected molecular weight ~72 kDa.
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Western blot testing of 1) rat lung, 2) rat skeletal muscle, 3) mouse lung and 4) mouse NIH 3T3 lysate with HIF3A antibody. Expected molecular weight ~72 kDa.
Western blot testing of human 1) HeLa, 2) A431, 3) Caco-2, 4) U-87 MG, 5) U-2 OS, 6) K562 and 7) PC-3 lysate with HIF3A antibody. Expected molecular weight ~72 kDa.
Flow cytometry testing of human U-87 MG cells with HIF3A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HIF3A antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q9Y2N7
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This HIF3A antibody is available for research use only.
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Description

Hypoxia-inducible factor 3 alpha, also called MOP7, is a protein that in humans is encoded by the HIF3A gene. HIF3A is mapped to 19q13.32. This gene involved in adaptive response to hypoxia. HIF3A suppresses hypoxia-inducible expression of HIF1A and EPAS1. It binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. This gene functions as an inhibitor of angiogenesis in the cornea.

Application Notes

Optimal dilution of the HIF3A antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids Q29-Q480) was used as the immunogen for the HIF3A antibody.

Storage

After reconstitution, the HIF3A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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