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Home >> Antibodies >> HIF1a Antibody [Discontinued, view alternative antibodies]

HIF1a Antibody [Discontinued, view alternative antibodies] (R30218)

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  Catalog No Formulation Size Price (USD)  
Image R30218 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
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Western blot testing of human 1) U-87 MG, 2) U-2 OS, 3) PC-3, 4) A549, 5) HeLa, 6) T-47D, 7) HepG2 and 8) Caco-2 cell lysate with HIF1a antibody at 0.5ug/ml. Routinely observed molecular weight: 100~120 kDa.
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Western blot testing of human 1) U-87 MG, 2) U-2 OS, 3) PC-3, 4) A549, 5) HeLa, 6) T-47D, 7) HepG2 and 8) Caco-2 cell lysate with HIF1a antibody at 0.5ug/ml. Routinely observed molecular weight: 100~120 kDa.
Western blot testing of HIF1a antibody and Lane 1: MCF-7; 2: HeLa; 3: Jurkat; 4: SMMC-7721; 5: HT1080 cell lysate. Routinely observed molecular weight: 100~120 kDa.
Availability Discontinued
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt Q16665
Localization Nuclear, possible cytoplasmic
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Limitations This HIF1a antibody is available for research use only.
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Description

Hypoxia-inducible factor 1 alpha is a transcription factor that mediates cellular and systemic homeostatic responses to reduced availability in mammals, including angiogenesis, erythropoiesis and glycolysis. This gene was mapped to 14q21-q24. HIF1a transactivate genes required for energy metabolism and tissue perfusion and is necessary for embryonic development and tumor explant growth. It is over expressed during carcinogenesis, myocardial infarction and wound healing. It is crucial for the cellular response to hypoxia and is frequently over expressed in human cancers, resulting in the activation of genes essential for cell survival. HIF1a regulates the survival and function in the inflammatory microenvironment directly. It is a transcription factor that plays a pivotal role in cellular adaptation to changes in oxygen availability.

Application Notes

The stated application concentrations are suggested starting points. Titration of the HIF1a antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids 8-21 (NDKKKISSERRKEK-human) were used as the immunogen for this HIF1a antibody.

Storage

After reconstitution, the HIF1a antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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