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Home >> Antibodies >> HIF1A Antibody

HIF1A Antibody (F54409)

  Catalog No Formulation Size Price (USD)  
Image F54409-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 359
Image
F54409-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 149
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt Q16665
Applications Flow cytometry : 1:25 (1x10e6 cells)
Western blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:25
Limitations This HIF1A antibody is available for research use only.
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Description

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas.

Application Notes

The stated application concentrations are suggested starting points. Titration of the HIF1A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Recombinant human protein was used as the immunogen for the HIF1A antibody.

Storage

Aliquot the HIF1A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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