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Home >> Antibodies >> HIF-2-alpha Antibody

HIF-2-alpha Antibody (R30297)

  Catalog No Formulation Size Price (USD)  
Image R30297 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of HIF-2 alpha antibody and rat brain tissue lysate
IHC-P: HIF-2-alpha antibody testing of rat small intestine tissue
Availability 1-3 business days
Species Reactivity Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt Q9JHS1
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Limitations This HIF-2-alpha antibody is available for research use only.
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Description

HIF-2-alpha is the protein product of the gene EPAS1. The human EPAS1 gene contains 15 exons and spans at least 120 kb. The positions of the introns within the genomic region encoding the N-terminal bHLH-PAS domains of EPAS1 and AHR are similar, suggesting that the 5-prime ends of the two genes may have arisen from a gene duplication event. HIF-2-alpha is predominantly expressed in highly vascularized tissues of adult humans and in endothelial cells of the mouse adult and embryo and may represent an important regulator of vascularization, perhaps involving the regulation of endothelial cell gene expression in response to hypoxia.

Application Notes

Titration of the HIF-2-alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids 202-240 (YNNCPPHSSLCGYKEPLLSCLIIMCEPIQHPSHMDIPLD-rat) were used as the immunogen for this HIF-2-alpha antibody.

Storage

The lyophilized HIF-2-alpha antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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