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Home >> Antibodies >> HIF-1 beta Antibody

HIF-1 beta Antibody (R31550)

  Catalog No Formulation Size Price (USD)  
Image R31550 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of HIF-1 beta antibody and Lane 1: HeLa; 2: 293T; 3: Jurkat; 4: U87; Lane5: COLO320. Expected/observed size ~87KD
Western blot testing of HIF-1 beta antibody and recombinant human protein (0.5ng)
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Gene ID 405
Applications Western blot : 0.5-1ug/ml
Limitations This HIF-1 beta antibody is available for research use only.
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Description

HIF-1 beta is also known as ARNT or HIF1B. This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. It is mapped to 1q21.3. The protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. HIF-1 beta is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. It is a structural component of the XRE-binding form of the Ah receptor. It also functions in concert with RelB in a CD30-induced negative feedback mechanism.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the HIF-1 beta antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 416-789) was used as the immunogen for this HIF-1 beta antibody.

Storage

After reconstitution, the HIF-1 beta antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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