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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
HIF-1 alpha Antibody / HIF1A (R31666)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
R31666 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 449 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat |
| Format | Antigen affinity purified |
| Host | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Antigen affinity |
| Buffer | Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide |
| Gene ID | 3091 |
| Localization | Nuclear, possible cytoplasmic |
| Applications | Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 0.5-1ug/ml |
| Limitations | This HIF-1 alpha antibody is available for research use only. |
|
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HIF-1 alpha Antibody recognizes Hypoxia inducible factor 1 subunit alpha, the oxygen-sensitive regulatory component of the HIF-1 transcription factor complex encoded by the HIF1A gene. HIF-1 alpha antibody, also widely referred to as HIF1A antibody and Hypoxia inducible factor 1 alpha antibody in the literature, detects a master regulator of cellular responses to hypoxia. Under normoxic conditions, HIF-1 alpha is rapidly hydroxylated and targeted for proteasomal degradation. In contrast, reduced oxygen tension stabilizes HIF-1 alpha, allowing it to accumulate in the nucleus and dimerize with HIF-1 beta to activate hypoxia-responsive genes.
Hypoxia inducible factor 1 subunit alpha functions as a transcription factor that regulates genes involved in angiogenesis, glycolysis, erythropoiesis, cell survival, and metabolic adaptation. HIF-1 alpha antibody is commonly used to evaluate hypoxic signaling pathways and tumor microenvironment biology, as HIF1A-driven transcription induces vascular endothelial growth factor, glucose transporters such as GLUT1, and multiple glycolytic enzymes. Nuclear localization of HIF-1 alpha is a hallmark of transcriptionally active hypoxia signaling in immunohistochemical studies.
HIF-1 alpha protein stability is tightly controlled by prolyl hydroxylases that modify specific proline residues in an oxygen-dependent manner. Hydroxylated HIF-1 alpha is recognized by the von Hippel-Lindau tumor suppressor complex and targeted for ubiquitin-mediated degradation. In hypoxia or in tumors with VHL pathway alterations, this degradation pathway is impaired, resulting in accumulation of HIF-1 alpha and activation of hypoxia responsive element-containing genes. HIF-1 alpha Antibody is therefore frequently applied in studies of renal cell carcinoma, glioblastoma, breast cancer, and other solid tumors characterized by hypoxic regions.
Beyond oncology, HIF-1 alpha plays important roles in ischemic injury, cardiovascular disease, inflammatory responses, and metabolic reprogramming. HIF1A signaling influences immune cell function, including macrophage polarization and T cell metabolism under low oxygen conditions. Cytoplasmic staining may reflect stabilized but not yet translocated protein, whereas nuclear staining is typically interpreted as active transcriptional engagement. HIF-1 alpha Antibody provides a valuable tool for assessing hypoxia pathway activation, angiogenic signaling, and metabolic adaptation in both basic research and translational studies.
By targeting a central mediator of oxygen sensing and cellular adaptation, HIF-1 alpha Antibody supports investigations into tumor hypoxia, therapeutic resistance, ischemia, and stress response pathways.
Titration of the HIF-1 alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Amino acids 703-732 (EEELNPKILALQNAQRKRKMEHDGSLFQAV-human) were used as the immunogen for this HIF-1 alpha antibody.
The lyophilized HIF-1 alpha antibody can be stored at 4-20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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