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Home >> Antibodies >> HIF-1 alpha Antibody (N-Terminal Region)

HIF-1 alpha Antibody (N-Terminal Region) (F54115)

  Catalog No Formulation Size Price (USD)  
F54115-0.2ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.2 ml 299

F54115-0.05ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.05 ml 125
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Western blot analysis of lysates from the human Jurkat cell line, untreated or treated with CoCl2 (0.1mM), using HIF-1 alpha antibody (upper) or Tubulin Ab (lower). Routinely observed molecular weight: 100~120 kDa.
Western blot analysis of lysates from the human Jurkat cell line, untreated or treated with CoCl2 (0.1mM), using HIF-1 alpha antibody (upper) or Tubulin Ab (lower). Routinely observed molecular weight: 100~120 kDa.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse, Rat, Bovine
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt Q16665
Localization Nuclear, possible cytoplasmic
Applications Western Blot : 1:1000
Limitations This HIF-1 alpha antibody is available for research use only.
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Description

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia. [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the HIF-1 alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 9-33 from human HIF1A was used as the immunogen for the HIF-1 alpha antibody.

Storage

Aliquot the HIF-1 alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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