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Home >> Antibodies >> HIF-1 alpha Antibody

HIF-1 alpha Antibody (F50744)

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  Catalog No Formulation Size Price (USD)  
Image F50744-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F50744-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of HIF-1 alpha antibody and MDA-MB231 lysate. Routinely observed molecular weight: 100~120 kDa.
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Western blot analysis of HIF-1 alpha antibody and MDA-MB231 lysate. Routinely observed molecular weight: 100~120 kDa.
Western blot analysis of HIF-1 alpha antibody and mouse cerebellum tissue lysate
Confocal immunofluorescent analysis of HIF-1 alpha antibody with MDA-MB231 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
IHC analysis of FFPE human hepatocarcinoma tissue stained with HIF-1 alpha antibody
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt Q16665
Localization Nuclear, possible cytoplasmic
Applications Western blot : 1:1000
Immunofluorescence : 1:10-1:50
IHC (Paraffin) : 1:10-1:50
Limitations This HIF-1 alpha antibody is available for research use only.
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Description

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT).

Application Notes

Titration of the HIF-1 alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 728-757 from the human protein was used as the immunogen for this HIF-1 alpha antibody.

Storage

Aliquot the HIF-1 alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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