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Home >> Antibodies >> HGS Antibody / HRS / HGF-regulated tyrosine kinase substrate

HGS Antibody / HRS / HGF-regulated tyrosine kinase substrate (RQ5980)

  Catalog No Formulation Size Price (USD)  
Image RQ5980 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human rectal cancer with HGS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with HGS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human testicular cancer with HGS antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human HeLa cells with HGS antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) rat brain, 2) rat PC-12, 3) rat NRK, 4) mouse brain, 5) mouse HEPA1-6, 6) mouse NIH 3T3 and 7) mouse RAW264.7 lysate with HGS antibody. Predicted molecular weight ~86 kDa, commonly observed at 110-115 kDa.
Flow cytometry testing of human A549 cells with HGS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HGS antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt O14964
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry : 1-2ug/ml
Immunofluorescence : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This HGS antibody is available for research use only.
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Description

Hepatocyte growth factor-regulated tyrosine kinase substrate is an enzyme that in humans is encoded by the HGS gene. It is mapped to 17q25.3. The protein encoded by this gene regulates endosomal sorting and plays a critical role in the recycling and degradation of membrane receptors. The encoded protein sorts monoubiquitinated membrane proteins into the multivesicular body, targeting these proteins for lysosome-dependent degradation.

Application Notes

Optimal dilution of the HGS antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids R3-D777) was used as the immunogen for the HGS antibody.

Storage

After reconstitution, the HGS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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