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Home >> Antibodies >> HGPRTase Antibody / HPRT1

HGPRTase Antibody / HPRT1 (RQ8055)

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  Catalog No Formulation Size Price (USD)  
Image RQ8055 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of fixed and permeabilized human U-2 OS cells with HGPRTase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HGPRTase antibody.
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Flow cytometry testing of fixed and permeabilized human U-2 OS cells with HGPRTase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HGPRTase antibody.
Flow cytometry testing of fixed and permeabilized human 293T cells with HGPRTase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HGPRTase antibody.
Western blot testing of 1) human HeLa, 2) human 293T, 3) human Jurkat, 4) rat brain and 5) mouse brain tissue lysate with HGPRTase antibody. Predicted molecular weight ~24 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P00492
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This HGPRTase antibody is available for research use only.
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Description

Hypoxanthine-guanine phosphoribosyltransferase (HGPRT, HGPRTase) is an enzyme encoded in humans by the HPRT1 gene. The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.

Application Notes

Optimal dilution of the HGPRTase antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids Y17-K186) was used as the immunogen for the HGPRTase antibody.

Storage

After reconstitution, the HGPRTase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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