HGPRTase Antibody / HPRT1 (RQ8055)

Description

HGPRTase (Hypoxanthine-guanine phosphoribosyltransferase, HPRT1) is a purine salvage enzyme that catalyzes the conversion of hypoxanthine to inosine monophosphate (IMP) and guanine to guanosine monophosphate (GMP) using phosphoribosyl pyrophosphate (PRPP) as a substrate. This reaction conserves energy by recycling purine bases into usable nucleotides, supporting DNA and RNA synthesis without the need for de novo purine biosynthesis.

HGPRTase is ubiquitously expressed in human tissues and is essential for maintaining balanced nucleotide pools. It plays a particularly important role in cells with high nucleic acid turnover, such as lymphocytes and neuronal cells. The enzyme is widely studied in research focused on nucleotide metabolism, purine salvage pathways, and enzymatic regulation of biosynthetic flux.

The HGPRTase antibody enables specific detection of endogenous HGPRT in applications such as western blot, immunohistochemistry, and immunofluorescence. Researchers use the HGPRTase antibody from NSJ Bioreagents to quantify expression levels, examine tissue distribution, and study the enzyme’s regulation under various metabolic conditions. With high specificity and consistent performance, the HGPRTase antibody supports detailed investigations into purine metabolism and nucleotide homeostasis.

Application Notes

Optimal dilution of the HGPRTase antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids Y17-K186) was used as the immunogen for the HGPRTase antibody.

Storage

After reconstitution, the HGPRTase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.