HGF-regulated tyrosine kinase substrate Antibody / HRS / HGS [clone 4B7E2] (RQ6925)

Immunofluorescent staining of FFPE human U-87 MG cells with HGF-regulated tyrosine kinase substrate antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

IHC staining of FFPE human endometrial cancer tissue with HGF-regulated tyrosine kinase substrate antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with HGF-regulated tyrosine kinase substrate antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human ovarian cancer tissue with HGF-regulated tyrosine kinase substrate antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human bladder epithelial carcinoma tissue with HGF-regulated tyrosine kinase substrate antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast infiltrating ductal carcinoma tissue with HGF-regulated tyrosine kinase substrate antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colorectal adenocarcinoma tissue with HGF-regulated tyrosine kinase substrate antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE mouse kidney tissue with HGF-regulated tyrosine kinase substrate antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of 1) human HeLa, 2) human 293T, 3) human K562, 4) human SK-O-V3, 5) rat brain, 6) rat PC-12, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with HGF-regulated tyrosine kinase substrate antibody. Predicted molecular weight ~86 kDa, commonly observed at 110-115 kDa.

Flow cytometry testing of human Caco-2 cells with HGF-regulated tyrosine kinase substrate antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HGF-regulated tyrosine kinase substrate antibody.