HFE Antibody / Hereditary Hemochromatosis Protein Antibody (R32770)

HFE Antibody Human Pancreatic Cancer High Magnification IHC. Immunohistochemical analysis of FFPE human pancreatic cancer tissue following heat-induced epitope retrieval in pH8 EDTA buffer. Tissue sections were incubated with HFE Antibody at 2 ug/ml overnight, followed by an HRP-conjugated secondary antibody and DAB chromogen for visualization. This higher magnification image highlights moderate membranous and cytoplasmic staining in the neoplastic epithelial cells, consistent with the localization of HFE as a membrane-associated regulator of iron homeostasis. These results demonstrate that this HFE Antibody, a Hereditary Hemochromatosis Protein Antibody, is suitable for detecting endogenous HFE expression in human FFPE pancreatic cancer tissue by immunohistochemistry.

HFE Antibody Human Pancreatic Cancer IHC. Immunohistochemical analysis of FFPE human pancreatic cancer tissue following heat-induced epitope retrieval in pH8 EDTA buffer. Tissue sections were incubated with HFE Antibody at 2 ug/ml overnight, followed by an HRP-conjugated secondary antibody and DAB chromogen for visualization. Moderate membranous and cytoplasmic staining is observed in the neoplastic epithelial cells, consistent with the localization of HFE as a membrane-associated regulator of iron homeostasis. This staining pattern demonstrates that this HFE Antibody, a Hereditary Hemochromatosis Protein Antibody, is suitable for detecting endogenous HFE expression in human FFPE pancreatic cancer tissue by immunohistochemistry.

HFE Antibody Human Fallopian Tube IHC. Immunohistochemical analysis of FFPE human fallopian tube tissue following heat-induced epitope retrieval in pH8 EDTA buffer. Tissue sections were incubated with HFE Antibody at 2 ug/ml overnight, followed by an HRP-conjugated secondary antibody and DAB chromogen for visualization. Moderate membranous and cytoplasmic staining is observed in the epithelial cells lining the mucosal folds, consistent with the localization of HFE as a membrane-associated regulator of iron homeostasis. This staining pattern demonstrates that this HFE Antibody, a Hereditary Hemochromatosis Protein Antibody, is suitable for detecting endogenous HFE expression in human FFPE tissue by immunohistochemistry.

HFE Antibody A549 Cell IF. Immunofluorescence analysis of A549 cells following enzymatic antigen retrieval. Cells were incubated with HFE Antibody at 5 ug/ml overnight, followed by a Cy3-conjugated secondary antibody. Nuclei were counterstained with DAPI (blue). HFE staining (red) is observed predominantly in the cytoplasm with membranous enrichment, consistent with the localization of this membrane-associated protein involved in iron sensing and systemic iron homeostasis. This staining pattern demonstrates that this HFE Antibody, a Hereditary Hemochromatosis Protein Antibody, is suitable for visualizing endogenous HFE expression and subcellular distribution in cultured cells by immunofluorescence.

HFE Antibody Human Epithelial Cell WB. Western blot analysis of HFE was performed using lysates from human Caco-2, HeLa, and A431 cells. A prominent band is detected at approximately 48-50 kDa in each cell line. Although the predicted molecular weight of HFE is approximately 40 kDa, the protein is commonly observed at a higher apparent molecular weight due to N-linked glycosylation, a post-translational modification required for proper folding, association with beta-2 microglobulin, and cell surface expression. These results demonstrate that this HFE Antibody, a Hereditary Hemochromatosis Protein Antibody, specifically detects endogenous HFE in multiple human epithelial cell lines by Western blot.