- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
HEXIM2 Antibody / Hexamethylene bisacetamide-inducible protein 2 (FY13208)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
FY13208 | Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml | 100 ug | 439 |
HEXIM2 antibody detects Hexamethylene bisacetamide-inducible protein 2, a transcriptional regulator that modulates RNA polymerase II elongation through control of P-TEFb (positive transcription elongation factor b) activity. The UniProt recommended name is Hexamethylene bisacetamide-inducible protein 2 (HEXIM2). This nuclear protein functions similarly to its paralog HEXIM1, acting as a negative regulator of transcriptional elongation by forming inhibitory complexes with cyclin-dependent kinase 9 (CDK9) and cyclin T1.
Functionally, HEXIM2 antibody identifies a 286-amino-acid protein localized predominantly in the nucleus, where it binds 7SK small nuclear RNA (7SK snRNA) to form the 7SK snRNP complex. This complex sequesters and inhibits P-TEFb, thereby controlling the phosphorylation of the C-terminal domain (CTD) of RNA polymerase II and modulating gene expression rates. HEXIM2 dynamically regulates the transition between transcriptional pausing and elongation, coordinating cellular responses to stress, differentiation, and growth factors.
The HEXIM2 gene is located on chromosome 17q21.32 and is expressed in various tissues, including brain, heart, and skeletal muscle. It plays important roles in transcriptional control during development and cell cycle progression, as well as in regulating expression of immediate early genes and viral transcriptional machinery.
Pathologically, altered HEXIM2 expression has been implicated in cancer, cardiac hypertrophy, and neurodegenerative diseases. Decreased HEXIM2 function enhances P-TEFb activity, leading to hyperactive transcription and dysregulated cell growth, while increased expression contributes to transcriptional repression and altered differentiation. Research using HEXIM2 antibody supports studies in transcriptional regulation, RNA biology, and disease-related gene expression control.
HEXIM2 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect nuclear transcriptional regulators. NSJ Bioreagents provides HEXIM2 antibody reagents optimized for studies in transcription elongation, gene expression, and cell signaling pathways.
Structurally, Hexamethylene bisacetamide-inducible protein 2 contains an RNA-binding domain and basic regions essential for 7SK snRNA interaction and P-TEFb inhibition. Its conformational flexibility allows rapid response to signaling cues that release or restore inhibition of transcription elongation. This antibody enables detailed investigation of HEXIM2s function in controlling RNA polymerase II-mediated transcription and cellular adaptation to stress.
Optimal dilution of the HEXIM2 antibody should be determined by the researcher.
E.coli-derived mouse HEXIM2 recombinant protein (Position: L17-R313) was used as the immunogen for the HEXIM2 antibody.
After reconstitution, the HEXIM2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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