HES5 Antibody / Hairy and Enhancer of Split 5 Polyclonal Antibody (RQ6640)

HES5 Antibody by IHC in human spleen. Immunohistochemistry of FFPE human spleen tissue using HES5 Antibody RQ6640. Brown immunoreactivity is distributed throughout the tissue, with staining visible in numerous cells across the examined section. Hematoxylin counterstains nuclei blue. Heat-induced epitope retrieval was performed by boiling sections in EDTA buffer, pH 8, for 20 minutes, followed by cooling.

HES5 Antibody by IHC in human gallbladder adenosquamous carcinoma. Immunohistochemistry of FFPE human gallbladder adenosquamous carcinoma using HES5 Antibody RQ6640. Brown staining is evident in the malignant epithelial cells forming irregular glandular structures, with both nuclear and cytoplasmic immunoreactivity visible. Surrounding stromal cells exhibit comparatively limited staining. Hematoxylin counterstains nuclei blue. Antigen retrieval was performed using EDTA buffer, pH 8, for 20 minutes at boiling temperature.

HES5 Antibody by IHC in human bladder epithelial carcinoma. Immunohistochemistry of FFPE human bladder epithelial carcinoma using HES5 Antibody RQ6640. Brown immunoreactivity is visible throughout the carcinoma cell clusters, with staining involving the cytoplasm and portions of the nuclear regions. The surrounding connective tissue shows less prominent staining. Hematoxylin counterstains nuclei blue. Heat-induced epitope retrieval was performed in EDTA buffer, pH 8, for 20 minutes.

HES5 Antibody by IHC in human placenta at higher magnification. Immunohistochemistry of FFPE human placental tissue using HES5 Antibody RQ6640. Brown staining outlines the trophoblast layer surrounding chorionic villi, with additional immunoreactivity visible within portions of the villous tissue. Hematoxylin counterstains nuclei blue. Heat-induced epitope retrieval was performed by boiling sections in EDTA buffer, pH 8, for 20 minutes.

HES5 Antibody by IHC in human placental chorionic villi. Immunohistochemistry of FFPE human placental tissue using HES5 Antibody RQ6640. Multiple chorionic villi exhibit brown staining along their peripheral trophoblast layers, while the central villous regions show more variable immunoreactivity. Hematoxylin counterstains nuclei blue. Heat-induced epitope retrieval was performed using EDTA buffer, pH 8, for 20 minutes at boiling temperature.

HES5 Antibody by WB in HaCaT and HepG2 cells. Western blot analysis of human HaCaT keratinocyte (lane 1) and HepG2 hepatocellular carcinoma (lane 2) cell lysates using HES5 Antibody RQ6640. A prominent immunoreactive band is detected near 18 kDa in both samples, consistent with the predicted molecular weight of HES5. No prominent additional bands are visible within the displayed molecular weight range.

HES5 Antibody by FACS in human PC-3 cells. Flow cytometry analysis of human PC-3 prostate carcinoma cells using HES5 Antibody RQ6640 at 1 ug per million cells, following blocking with goat serum. The red histogram represents cells alone, green represents the isotype control, and blue represents HES5 antibody staining. The blue fluorescence distribution is shifted to the right of the isotype control, indicating increased antibody-associated fluorescence.