HER4 Antibody Rabbit Polyclonal / ERBB4 (RQ8802)

Immunohistochemistry of HER4 antibody in human pancreas ductal adenocarcinoma tissue. FFPE human pancreatic ductal adenocarcinoma sections were subjected to heat-induced epitope retrieval by boiling in pH 8 EDTA for 20 minutes followed by cooling prior to staining. The rabbit polyclonal HER4 antibody was used as the detecting antibody. HRP-DAB brown staining is observed predominantly along the membranous and cytoplasmic compartments of malignant ductal epithelial cells, while surrounding stromal elements show comparatively lower background staining. The staining pattern is consistent with Erb-B2 receptor tyrosine kinase 4 expression in pancreatic carcinoma tissue.

IHC staining of FFPE human pancreas ductal adenocarcinoma tissue with HER4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunohistochemistry of HER4 antibody in human colon adenocarcinoma tissue. FFPE human colon adenocarcinoma sections were subjected to heat-induced epitope retrieval by boiling in pH 8 EDTA for 20 minutes followed by cooling prior to staining. The rabbit polyclonal HER4 antibody was used as the detecting antibody. HRP-DAB brown staining is observed predominantly in the membranous and cytoplasmic compartments of malignant glandular epithelial cells, while adjacent stromal elements display comparatively lower background staining. The staining pattern is consistent with Erb-B2 receptor tyrosine kinase 4 expression in colon adenocarcinoma tissue.

IHC staining of FFPE human colon adenocarcinoma tissue with HER4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human placental tissue with HER4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Immunohistochemistry of HER4 antibody in human placental tissue. FFPE human placenta sections were subjected to heat-induced epitope retrieval by boiling in pH 8 EDTA for 20 minutes followed by cooling prior to staining. The rabbit polyclonal HER4 antibody was used as the detecting antibody. HRP-DAB brown staining is observed predominantly along the membranous borders of trophoblastic cells with additional cytoplasmic signal, while surrounding stromal components display lower background staining. The staining pattern is consistent with Erb-B2 receptor tyrosine kinase 4 expression in placental tissue.

IHC staining of FFPE human urothelial carcinoma tissue with HER4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human urothelial carcinoma tissue with HER4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of 1) human MCF7, 2) rat brain, 3) rat PC-12 and 4) mouse brain tissue lysate with HER4 antibody. Predicted molecular weight: 147-180kDa (precursor), 120, 80 kDa (cleaved forms).