HER4 Antibody Protein Microarray Validated / ERBB4 [clone ERBB4/2581] (V7740)

Immunohistochemistry of protein microarray validated HER4 antibody in human breast carcinoma tissue. FFPE human breast carcinoma sections were subjected to heat-induced epitope retrieval by boiling in 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 minutes followed by cooling at room temperature prior to staining. The mouse monoclonal HER4 antibody clone ERBB4/2581 was used as the detecting antibody and is validated by protein microarray analysis. HRP-DAB brown staining is observed predominantly along the membranous and cytoplasmic compartments of tumor epithelial cells, while surrounding stromal elements show comparatively reduced background staining. The staining pattern is consistent with Erb-B2 receptor tyrosine kinase 4 expression in breast carcinoma tissue.

IHC staining of FFPE human breast carcinoma with protein microarray validated HER4 antibody (clone ERBB4/2581). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT prior to testing.

HuProt human protein microarray specificity analysis of HER4 antibody clone ERBB4/2581. The protein microarray validated monoclonal antibody was screened against more than 19,000 full-length human proteins. ERBB4 ranked as the top hit with the highest signal intensity, demonstrating strong target specificity relative to other proteins on the array.
Z-score represents the strength of the signal generated when the antibody, together with a fluorescently labeled anti-IgG secondary antibody, binds to a specific protein on the HuProt array. Z-scores are expressed in units of standard deviations above the mean signal of all proteins on the array. When proteins are ranked in descending order by Z-score, the S-score reflects the difference in standard deviations between adjacent ranked proteins. The S-score therefore indicates the relative specificity of the antibody for its intended target compared with other proteins on the array.

SDS-PAGE analysis of purified, BSA-free HER4 antibody (clone ERBB4/2581) as confirmation of integrity and purity.