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Home >> Antibodies >> HER2 ErbB2 Antibody

HER2 ErbB2 Antibody [clone HRB2/282] (V2110)

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  Catalog No Formulation Size Price (USD)  
Image V2110-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 539
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V2110-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 249
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V2110SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using HER2 ErbB2 antibody (clone HRB2/282). These results demonstrate the foremost specificity of the HRB2/282 mAb.Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using HER2 ErbB2 antibody (clone HRB2/282). These results demonstrate the foremost specificity of the HRB2/282 mAb.Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Immunofluorescent staining of PFA-fixed human MCF7 cells with HER2 ErbB2 antibody (clone HRB2/282, green) and Reddot nuclear stain (red).
Flow cytometry testing of PFA-fixed human MCF7 cells with HER2 ErbB2 antibody (clone HRB2/282); Red=isotype control, Blue= HER2 ErbB2 antibody.
SDS-PAGE analysis of purified, BSA-free HER2 ErbB2 antibody (clone HRB2/282) as confirmation of integrity and purity.
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name HRB2/282
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 2064
Localization Extracellular/Intracellular cell membrane (this mAb is binds to the extracellular portion of the protein)
Applications ELISA : order BSA/sodium azide-free format for coating
Flow cytometry : 0.5-1ug/million cells
Immunofluorescence : 0.5-1ug/ml
Limitations This HER2 ErbB2 antibody is available for research use only.
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Description

This antibody recognizes a protein of 185kDa, which is identified as HER2 / ErbB2. Its epitope is localized in the extracellular domain. HER2 is a member of the EGFR family. This antibody is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. HER2 / ErbB2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. This HER2 antibody binds to the extracellular/cell surface region of the protein.

Immunogen

Recombinant human HER2 protein was used as the immunogen for this antibody.(1) Its epitope is localized in the extracellular domain.

Storage

Store the HER2 ErbB2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

p185, CD340, Verb b2 Erythroblastic Leukemia Viral Oncogene Homolog 2, ErbB2 antibody, Neuro/Glioblastoma Derived Oncogene Homolog, HER2 antibody

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