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Home >> Antibodies >> HER2 Antibody / ErbB2

HER2 Antibody / ErbB2 [clone SPM172] (V7729)

  Catalog No Formulation Size Price (USD)  
Image V7729-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7729-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7729SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human breast carcinoma with HER2 antibody (clone SPM172). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free HER2 antibody (clone SPM172) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG
Clone Name SPM172
Purity Protein G affinity chromatography
UniProt P04626
Localization Cell surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This HER2 antibody is available for research use only.
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Description

This MAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Application Notes

Optimal dilution of the HER2 antibody should be determined by the researcher.

Immunogen

A recombinant human HER2 protein fragment within amino acids 311-462 was used as the immunogen for the HER2 antibody.

Storage

Store the HER2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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