HER2 Antibody / ErbB2 / Extracellular region [clone ERBB2/4439R] (V8633)

IHC staining of FFPE human breast carcinoma with HER2 antibody (clone ERBB2/4439R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast carcinoma with HER2 antibody (clone ERBB2/4439R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of PFA-fixed human MCF7 cells with HER2 antibody (clone ERBB2/4439R, green) and Phalloidin (red).

Flow cytometry testing of PFA-fixed human MCF7 cells with HER2 antibody (clone ERBB2/4439R); Red=isotype control, Blue= HER2 antibody.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using HER2 antibody. These results demonstrate the foremost specificity of the ERBB2/4439R mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.