HER2 Antibody / Extracellular Domain Antibody [clone ERBB2/3078] (V7735)

HER2 Antibody Breast Carcinoma Membrane IHC. Immunohistochemistry analysis of FFPE human breast carcinoma tissue using HER2 Antibody (clone ERBB2/3078) demonstrates strong HRP-DAB brown membranous staining outlining tumor epithelial cells, consistent with detection of ErbB2 / HER2 within an extracellular region of this cell surface receptor, while surrounding stromal tissue shows minimal signal; nuclei are counterstained blue. HIER: boil tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min and allow to cool before testing.

HER2 Antibody Breast Tumor Surface IHC. Immunohistochemistry analysis of FFPE human breast carcinoma tissue using HER2 Antibody (clone ERBB2/3078) highlights continuous HRP-DAB brown membranous staining across tumor cell clusters, emphasizing cell surface localization of ErbB2 / HER2 consistent with an antibody generated against an extracellular portion of the receptor, while adjacent stromal regions remain largely negative; nuclei are counterstained blue. HIER: boil tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min and allow to cool before testing.

HER2 Antibody Breast Tumor Focal IHC. Immunohistochemistry analysis of FFPE human breast carcinoma tissue using HER2 Antibody (clone ERBB2/3078) demonstrates moderate to strong HRP-DAB brown membranous staining in focal tumor regions, consistent with variable ErbB2 / HER2 expression at the cell surface and detection within an extracellular region of the receptor, while surrounding stromal components show minimal background staining; nuclei are counterstained blue. HIER: boil tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min and allow to cool before testing.

HER2 Antibody Microarray Specificity Validation. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using HER2 Antibody (clone ERBB2/3078) demonstrates highly specific detection of ErbB2 / HER2, a cell surface receptor with an extracellular domain targeted by this antibody. The antibody shows a dominant signal for ERBB2 with clear separation from other proteins on the array, supporting strong target specificity of clone ERBB2/3078. Z- and S-score: The Z-score represents the strength of signal generated when the antibody binds to a protein on the array, expressed as standard deviations above the mean signal, while the S-score reflects the difference between sequential Z-scores and indicates relative specificity compared to potential off-target interactions.

SDS-PAGE analysis of purified, BSA-free HER2 antibody (clone ERBB2/3078) as confirmation of integrity and purity.