HER-2 Antibody / ErbB2 [clone ERBB2/3093] (V7877)

HER2 Antibody Breast Tumor Dense Membrane IHC. Immunohistochemistry analysis of FFPE human breast carcinoma tissue using HER-2 Antibody (clone ERBB2/3093) demonstrates intense, continuous HRP-DAB brown membranous staining in densely packed tumor cell regions, emphasizing high surface expression of ErbB2 / HER2 as a receptor tyrosine kinase, while surrounding stromal components show minimal background staining; nuclei are counterstained blue. HIER: boil tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min and allow to cool before testing.

HER2 Antibody Breast Carcinoma Tubular IHC. Immunohistochemistry analysis of FFPE human breast carcinoma tissue using HER-2 Antibody (clone ERBB2/3093) demonstrates strong HRP-DAB brown membranous staining along elongated, tubular tumor structures, consistent with ErbB2 / HER2 expression as a cell surface receptor, while surrounding stromal tissue shows minimal signal; nuclei are counterstained blue. HIER: boil tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min and allow to cool before testing.

HER2 Antibody Breast Tumor Patchy IHC. Immunohistochemistry analysis of FFPE human breast carcinoma tissue using HER-2 Antibody (clone ERBB2/3093) highlights patchy yet distinct HRP-DAB brown membranous staining across tumor cell groups, reflecting variable ErbB2 / HER2 expression within the tumor microenvironment, while adjacent stromal regions remain largely negative; nuclei are counterstained blue. HIER: boil tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min and allow to cool before testing.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using HER-2 antibody (clone ERBB2/3093). These results demonstrate the foremost specificity of the ERBB2/3093 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free HER-2 antibody (clone ERBB2/3093) as confirmation of integrity and purity.