- Tel: 858.663.9055
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
HER-2 Antibody [clone ERB2/776] (V2377)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
 |
V2377-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 519 | |
|
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V2377-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 229 | |
|
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V2377SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 519 |
| Species Reactivity | Human |
| Format | Purified |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | ERB2/776 |
| Purity | Protein G affinity chromatography |
| Buffer | 1X PBS, pH 7.4 |
| Gene ID | 2064 |
| Localization | Extracellular/Intracellular cell membrane (this mAb is binds to the intracellular portion of the protein) |
| Applications | ELISA : order BSA/sodium azide-free format for coating Flow cytometry : 1-2ug/million cells Immunofluorescence : 1-2ug/ml |
| Limitations | This HER-2 antibody is available for research use only. |
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ErbB2, also called HER2, is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. This ErbB2 antibody shows minimal cross-reaction with other members of the family. The protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HER-2 antibody to be titered up or down for optimal performance.
1. FFPE staining requires boiling tissue sections in 10mM Citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
2. This HER2 antibody is specific to the intracellular region of the protein.
Recombinant human protein (intracellular domain) was used as the immunogen for this HER-2 antibody. (2)
Store the HER-2 antibody at 2-8oC.
p185, CD340, Verb b2 Erythroblastic Leukemia Viral Oncogene Homolog 2, ErbB2 antibody, Neuro/Glioblastoma Derived Oncogene Homolog, HER2 antibody
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