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Home >> Antibodies >> HER-2 Antibody

HER-2 Antibody [clone ERB2/776] (V2377)

  Catalog No Formulation Size Price (USD)  
Image V2377-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2377-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2377SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
FFPE staining of human breast carcinoma with HER-2 antibody (ERB2/776).
SDS-PAGE Analysis of Purified, BSA-Free HER-2 Antibody (clone ERB2/776). Confirmation of Integrity and Purity of the Antibody.
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name ERB2/776
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 2064
Localization Extracellular/Intracellular cell membrane (this mAb is binds to the intracellular portion of the protein)
Applications ELISA (order BSA/sodium azide-free format for coating) :
Flow cytometry : 1-2ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Limitations This HER-2 antibody is available for research use only.
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Description

ErbB2, also called HER2, is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. This ErbB2 antibody shows minimal cross-reaction with other members of the family. The protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HER-2 antibody to be titered up or down for optimal performance.
1. FFPE staining requires boiling tissue sections in 10mM Citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
2. This HER2 antibody is specific to the intracellular region of the protein.

Immunogen

Recombinant human protein (intracellular domain) was used as the immunogen for this HER-2 antibody. (2)

Storage

Store the HER-2 antibody at 2-8°C.

Alternate Names

p185, CD340, Verb b2 Erythroblastic Leukemia Viral Oncogene Homolog 2, ErbB2 antibody, Neuro/Glioblastoma Derived Oncogene Homolog, HER2 antibody

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