Hepatoma-Derived Growth Factor Antibody PCRP-HDGF-1D1 / Nuclear Mitogen Antibody [clone PCRP-HDGF-1D1] (V4749)

Hepatoma-Derived Growth Factor Antibody PCRP-HDGF-1D1 in Human HeLa Cervical Cancer Cells FACS. Flow cytometry testing of PFA-fixed human HeLa cervical cancer cells with clone PCRP-HDGF-1D1 followed by goat anti-mouse IgG-CF488. Antibody-stained cells (blue) show a clear fluorescence shift relative to unstained cells (red), demonstrating detection of the nuclear mitogen HDGF in HeLa cells.

Hepatoma-Derived Growth Factor Antibody PCRP-HDGF-1D1 in Human Colon Carcinoma IHC. Immunohistochemistry testing of clone PCRP-HDGF-1D1 in FFPE human colon carcinoma tissue. HIER was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling before testing. Strong, predominantly nuclear immunoreactivity is observed in the carcinoma cells, consistent with the nuclear localization of HDGF.

Hepatoma-Derived Growth Factor Antibody PCRP-HDGF-1D1 Protein Microarray Validation. Protein microarray analysis of clone PCRP-HDGF-1D1 using a HuProt array containing more than 19,000 full-length human proteins. HDGF produces the dominant signal with an S-score of 36.63, showing substantial separation from the next-highest protein and supporting strong target specificity. The Z-score represents signal strength in standard deviations above the array mean, while the S-score represents separation between the intended target and the next-highest signal.

Purified Hepatoma-Derived Growth Factor Antibody PCRP-HDGF-1D1 SDS-PAGE. Electrophoretic analysis of 2 ug of purified, BSA-free clone PCRP-HDGF-1D1 under non-reducing (NR) and reducing (R) conditions. The non-reduced sample shows the intact antibody at approximately 150 kDa, while reduction produces the expected immunoglobulin heavy and light chain bands at approximately 50 and 25 kDa, respectively, supporting the integrity and purity of the preparation.