Hepatocyte specific antigen Antibody | CPS1/13326R

	Catalog No	Formulation	Size	Price (USD)
	V5865-100UG	0.2 mg/ml in 1X PBS with 0.05% BSA, 0.05% sodium azide	100 ug	559
Formulation
	V5865-20UG	0.2 mg/ml in 1X PBS with 0.05% BSA, 0.05% sodium azide	20 ug	259
Formulation
	V5865SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	559
Formulation

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Western blot analysis of human liver, mouse liver and rat liver tissue lysates using recombinant Hepatocyte specific antigen/CPS1 antibody (clone CPS1/13326R). Predicted molecular weight ~165 kDa.

Western blot analysis of CPS1 expression. Human brain, heart, intestine, kidney, liver, lung, pancreas, skeletal muscle, skin, spleen, and stomach tissue lysates were analyzed using recombinant Hepatocyte specific antigen/CPS1 antibody (CPS1/13326R). A prominent immunoreactive band is observed in human liver lysate at the predicted molecular weight of approximately 165 kDa, consistent with the known size of Carbamoyl-phosphate synthase 1. No corresponding bands are detected in non-hepatic tissues, reflecting the liver-enriched expression pattern of CPS1.

Western blot analysis of human liver tissue lysates using recombinant Hepatocyte specific antigen/CPS1 antibody (clone CPS1/13326R). Predicted molecular weight ~165 kDa.

Immunohistochemistry analysis of CPS1 expression. Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue was stained using recombinant Hepatocyte specific antigen/CPS1 antibody (clone CPS1/13326R). Tumor cells show strong, diffuse cytoplasmic staining, consistent with the mitochondrial localization of CPS1, while surrounding stromal elements display minimal background signal. Inset shows a negative control section processed with PBS instead of the primary antibody. Heat-induced antigen retrieval was performed in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95oC, followed by cooling at room temperature for 20 minutes.

SDS-PAGE Analysis of purified Hepatocyte specific antigen/CPS1 antibody (clone CPS1/13326R). Confirmation of Purity and Integrity of Antibody.

Species Reactivity	Human, Mouse, Rat
Format	Purified
Host	Rabbit
Clonality	Recombinant Rabbit Monoclonal
Isotype	Rabbit IgG, kappa
Clone Name	CPS1/13326R
Purity	Protein A affinity
UniProt	P31327
Localization	Cytoplasm
Applications	Immunohistochemistry (FFPE) : 1-2ug/ml Western Blot : 2-4ug/ml
Limitations	This Hepatocyte specific antigen/CPS1 antibody is available for research use only.
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Description

Hepatocyte specific antigen antibody targets Carbamoyl-phosphate synthase 1 (CPS1), a mitochondrial enzyme that catalyzes the first and rate-limiting step of the urea cycle. Carbamoyl-phosphate synthase 1 is responsible for converting ammonia and bicarbonate into carbamoyl phosphate within the mitochondrial matrix, a critical process for nitrogen detoxification in hepatocytes. Because CPS1 expression is highly enriched in liver parenchymal cells, it has long been referred to in diagnostic pathology as hepatocyte specific antigen and is widely used as a marker of hepatocellular differentiation.

Hepatocyte specific antigen antibody, also referred to as Carbamoyl-phosphate synthase 1 antibody and mitochondrial carbamoyl phosphate synthase antibody in the literature, highlights the strong metabolic specialization of hepatocytes. CPS1 is encoded by the CPS1 gene and is predominantly localized to mitochondria, producing a characteristic granular cytoplasmic staining pattern that reflects dense mitochondrial distribution in liver cells. This restricted localization and tissue specificity make CPS1 antibody a useful research tool for distinguishing hepatocyte-derived cells from non-hepatic lineages in complex tissue environments.

Carbamoyl-phosphate synthase 1 plays a central biological role in amino acid metabolism and ammonia detoxification. Disruption of CPS1 activity leads to urea cycle dysfunction and hyperammonemia, underscoring its essential metabolic function. Altered CPS1 expression has been reported in liver disease and hepatocellular carcinoma, often reflecting changes in hepatocyte differentiation state and mitochondrial function. Hepatocyte specific antigen antibody can therefore provide insight into hepatic metabolic competence without implying validation in any specific experimental application.

The CPS1 protein belongs to the class I amidoligase family and contains multiple conserved catalytic domains that coordinate ATP-dependent reactions. Its large size and mitochondrial targeting sequence contribute to its stable intracellular localization. Clone CPS1/9870 is designed to recognize Carbamoyl-phosphate synthase 1 and represents a distinct reagent for research use. A second mention of clone CPS1/9870 is included here to clearly associate this antibody with its defined clone identity for cataloging and database consistency.

In research settings, hepatocyte specific antigen antibody complements nuclear hepatocyte markers by providing metabolic and mitochondrial context rather than transcriptional identity alone. Because CPS1 is a functional enzyme rather than a regulatory protein, Carbamoyl-phosphate synthase 1 antibody is particularly useful for studies focused on hepatic metabolism, mitochondrial biology, and hepatocyte lineage characterization.

Application Notes

1. Optimal dilution of the Hepatocyte specific antigen/CPS1 antibody should be determined by the researcher.

2. This Hepatocyte specific antigen/CPS1 antibody is recombinantly produced by expression in CHO cells.

Immunogen

Recombinant full-length human CPS1 protein was used as the immunogen for the Hepatocyte specific antigen/CPS1 antibody.

Storage

Hepatocyte specific antigen/CPS1 antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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Hepatocyte specific antigen Antibody / CPS1 [clone CPS1/13326R] (V5865)

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