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Home >> Antibodies >> Hemoglobin subunit gamma-2 Antibody / HBG2

Hemoglobin subunit gamma-2 Antibody / HBG2 (F54741)

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  Catalog No Formulation Size Price (USD)  
Image F54741-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54741-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC testing of FFPE human liver tissue with Hemoglobin subunit gamma-2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of FFPE human liver tissue with Hemoglobin subunit gamma-2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE mouse liver tissue with Hemoglobin subunit gamma-2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human brain tissue lysate with Hemoglobin subunit gamma-2 antibody. Predicted molecular weight ~16 kDa.
Flow cytometry testing of human U-87 MG cells with Hemoglobin subunit gamma-2 antibody; Blue=isotype control, Green= Hemoglobin subunit gamma-2 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
UniProt P69892
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1:25
Flow cytometry : 1:25 (1x10e6 cells)
Western blot : 1:500-1:1000
Limitations This Hemoglobin subunit gamma-2 antibody is available for research use only.
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Description

Gamma chains make up the fetal hemoglobin F, in combination with alpha chains. [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the Hemoglobin subunit gamma-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 101-133 from the human protein was used as the immunogen for the Hemoglobin subunit gamma-2 antibody.

Storage

Aliquot the Hemoglobin subunit gamma-2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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