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Home >> Antibodies >> Helicobacter Pylori Antibody

Helicobacter Pylori Antibody (V2340)

  Catalog No Formulation Size Price (USD)  
Image V2340-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2340-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2340SAF-100UG 1 mg/ml in 1X PBS with 0.05% sodium azide; BSA-free 100 ug 429
Formalin/paraffin human stomach stained with Helicobacter pylori antibody.
Species Reactivity Helicobacter pylori
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Protein A affinity chromatography
Buffer 1X PBS, pH 7.4
Localization Cell surface and cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Helicobacter pylori antibody is available for research use only.
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Description

The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. This bacteria is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual Helicobacter pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunogen

Total sonicate of Helicobacter ylori was used as the immunogen for this antibody.

Storage

Store the Helicobacter Pylori antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

H. pylori, Helicobacter Pylori Antibody

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