HE4 Antibody / WFDC2 (RQ4465)

HE4 Antibody Human, Mouse and Rat WB. Western blot analysis was performed using HE4 Antibody at 0.5 ug/mL. Lane 1: human HeLa cell lysate; lane 2: human MDA-MB-231 cell lysate; lane 3: human MDA-MB-451 cell lysate; lane 4: rat thymus lysate; lane 5: mouse testis lysate; lane 6: mouse thymus lysate. The predicted molecular weight of HE4 is approximately 13-25 kDa depending on the degree of glycosylation. A specific immunoreactive band is detected in human, rat, and mouse samples at the expected molecular weight range, demonstrating that this Human Epididymis Protein 4 Antibody recognizes HE4 across multiple species and is suitable for Western blot analysis.

HE4 Antibody Rat Small Intestine IHC. Immunohistochemistry was performed on FFPE rat small intestine tissue using HE4 Antibody at 0.5 ug/mL. Heat-induced epitope retrieval was carried out in pH 6 citrate buffer for 10-20 minutes, followed by cooling to room temperature before incubation with the primary antibody. Bound antibody was visualized using an HRP-conjugated secondary antibody and DAB chromogen. Distinct cytoplasmic staining is observed in cells within the intestinal tissue, consistent with HE4 expression, while surrounding tissue shows minimal background staining. These results demonstrate that this Human Epididymis Protein 4 Antibody is suitable for detecting HE4 in formalin-fixed, paraffin-embedded rat tissue by immunohistochemistry.

HE4 Antibody Rat Spleen IHC. Immunohistochemistry was performed on FFPE rat spleen tissue using HE4 Antibody at 0.5 ug/mL. Heat-induced epitope retrieval was carried out in pH 6 citrate buffer for 10-20 minutes, followed by cooling to room temperature before incubation with the primary antibody. Bound antibody was visualized using an HRP-conjugated secondary antibody and DAB chromogen. Distinct staining is observed in a limited population of splenic cells, while the majority of surrounding tissue remains negative, demonstrating selective HE4 expression with low background. These results support the use of this Human Epididymis Protein 4 Antibody for immunohistochemical detection of HE4 in formalin-fixed, paraffin-embedded rat tissues.

HE4 Antibody Mouse Spleen IHC. Immunohistochemistry was performed on FFPE mouse spleen tissue using HE4 Antibody at 0.5 ug/mL. Heat-induced epitope retrieval was carried out in pH 6 citrate buffer for 10-20 minutes, followed by cooling to room temperature before incubation with the primary antibody. Bound antibody was visualized using an HRP-conjugated secondary antibody and DAB chromogen. Distinct staining is observed in scattered splenic cells, while most surrounding lymphoid tissue remains negative, indicating selective HE4 expression with minimal background. These findings demonstrate that this Human Epididymis Protein 4 Antibody is suitable for immunohistochemical detection of HE4 in formalin-fixed, paraffin-embedded mouse tissue.